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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 927-941
DOI: https://doi.org/10.20546/ijcmas.2017.606.109


Characterization of β-Lactamase from Two Pathogenic Bacteria
Hamed M. El-Shora1, Huda S. Al-Hayanni* and Ahmed M. El-Shobaky1
1Botany Department, Faculty of Science, Mansoura University, Egypt
2Biology Department, College of Science for Women, University of Baghdad, Iraq
*Corresponding author
Abstract:

 Beta-lactamase (EC 3.5.2.6) was isolated and purified from two clinical isolates of Staphylococcus sciuri and Klebsiella pneumoniae by several steps included precipitation with ammonium sulphate at 80% saturation, DEAE-Cellulose and gel filtration on Sephadex G-200 column. The characterization of the purified β-lactamase showed that the molecular weight was 30 KDa for S. sciuri β-lactamase, and 28 KDa for purified K. pneumoniae β-lactamase as estimated by sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The purified enzyme from S. sciuri and K. pneumonae has an optimal temperatures of 35°C and 40°C, respectively. The enzyme from S. sciuri was more stable than that of K. pneumoniae. The optimal pH value were 7.0 and 6.0 from S. sciuri and K. pneumoniae, respectively. The best concentrations of penicillin G were 400 μg ml- and 500 μg ml-1 for the enzyme from S. sciuri and K. pneumoniae. The increase in the enzyme concentration resulted in continuous increase in its activity from both bacteria.


Keywords: β-lactamase, Staphylococcus sciuri, Klebsiella pneumoniae, Purification, Characterization, Kinetic parameters.
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How to cite this article:

Hamed M. El-Shora, Huda S. Al-Hayanni and Ahmed M. El-Shobaky. 2017. Characterization of β-Lactamase from Two Pathogenic Bacteria.Int.J.Curr.Microbiol.App.Sci. 6(6): 927-941. doi: https://doi.org/10.20546/ijcmas.2017.606.109