National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out to assess the relative expression of Y- specific genes [Sex determining region on Y-chromosome (SRY), Testis-specific protein Y-encoded (TSPY) and Ubiquitin specific peptidase 9-Y-linked (USP9Y)] and their association with semen production characters in crossbred Jersey bulls. The blood samples were collected from breeding bulls present in three frozen semen stations in Tamil Nadu. The expressions of Y-specific genes were quantified by quantitative real-time PCR (qPCR) using SYBR green chemistry. The relative standard curve method was used to study the expression of Y-specific genes relative to a reference gene DEAD box polypeptide 3-Y-chromosome (DDX3Y) as control. The increase in expression values of SRY gene was positively and significantly (P<0.01) correlated with semen volume (0.688) and initial sperm motility (0.739). The decrease in expression value of TSPY gene was associated with increase in semen volume, sperm concentration, initial sperm motility and post-thaw motility over the years, which was significant (P<0.01) and negatively correlated. The decrease in expression of USP9Y gene was associated with increase in initial sperm motility and post-thaw motility over the years.
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