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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 893-900
DOI: https://doi.org/10.20546/ijcmas.2017.606.105


Relative Quantification of Expression of Y-Specific Genes and its Association with Semen Production Traits in Crossbred Jersey Bulls
A. Gopinathan*, S.N. Sivaselvam, J. John Kirubaharan and S.M.K. Karthickeyan
Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai – 600 007, India
*Corresponding author
Abstract:

The present study was carried out to assess the relative expression of Y- specific genes [Sex determining region on Y-chromosome (SRY), Testis-specific protein Y-encoded (TSPY) and Ubiquitin specific peptidase 9-Y-linked (USP9Y)] and their association with semen production characters in crossbred Jersey bulls. The blood samples were collected from breeding bulls present in three frozen semen stations in Tamil Nadu. The expressions of Y-specific genes were quantified by quantitative real-time PCR (qPCR) using SYBR green chemistry. The relative standard curve method was used to study the expression of Y-specific genes relative to a reference gene DEAD box polypeptide 3-Y-chromosome (DDX3Y) as control. The increase in expression values of SRY gene was positively and significantly (P<0.01) correlated with semen volume (0.688) and initial sperm motility (0.739). The decrease in expression value of TSPY gene was associated with increase in semen volume, sperm concentration, initial sperm motility and post-thaw motility over the years, which was significant (P<0.01) and negatively correlated. The decrease in expression of USP9Y gene was associated with increase in initial sperm motility and post-thaw motility over the years.


Keywords: Relative Expression, Y-specific genes, Crossbred Jersey bulls and qPCR.
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How to cite this article:

Gopinathan A., S.N. Sivaselvam, J. John Kirubaharan and Karthickeyan S.M.K. 2017. Relative Quantification of Expression of Y-Specific Genes and Its Association with Semen Production Traits in Crossbred Jersey BullsInt.J.Curr.Microbiol.App.Sci. 6(6): 893-900. doi: https://doi.org/10.20546/ijcmas.2017.606.105