National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Endophytic bacteria to promote plant growth by facilitating nutrient acquisition through the fixation of nitrogen, solubilizing phosphate, producing siderophores, producing plant growth hormones, or enzyme1- aminocyclopropane-1- carboxylate (ACC) deaminase and protecting plants from pathogens, via production of antibacterial or antifungal agents, or outcompeting pathogens for nutrients. Isolation and development of new selected plant growth promoting endophytic bacterial strains could be one of the many new approaches that are needed to aid the growth and health of agricultural crops, to eliminate or minimize the harmful effects of inorganic fertilizers, and to conserve organic and inorganic soil nutrients. The aim of this study was to isolate, characterize and identify endophytic bacteria from plants growing along the stream banks in Hot Springs, South Dakota. The bacterial endophytes were isolated, identified and screened in vitrofor morphological features (Gram stain, Gram morphology, and colony morphology). Further, isolates exhibiting difference in morphological features were selected for molecular identification through partial 16S-rRNA gene sequencing. Twenty-five endophytic bacteria strains were isolated from monocotyledons plants, viz. Typha, Bromus tectorum and Festuca and eight strains from a dicotyledonous plant, Nasturtium officinale. All the isolated endophytic bacteria were identified as different bacterial strains belonging to Bacillus thuringensis, B. cereus, B. atrophaeus, Pseudomonas sp., Cedeceadavisae, Escherichia sp., Acinetobacter calcoaceticus, Lysinobacillus sp., Pantoea sp., and Citrobacter freundii. Further investigation is needed to screen these isolated endophytic bacteria for different activities known to promote plant growth and protection from phytopathogen.
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