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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 756-767
DOI: https://doi.org/10.20546/ijcmas.2017.606.089


Isolation, Characterization and Identification of Putative Bacterial Endophytes from Some Plants in Hot Springs, South Dakota
Mada Faisal Ashkan1* and Bruce Bleakley2
1Biological Science Department, King Abdulaziz University, Rabigh, Saudi Arabia
2Biology and Microbiology Department, South Dakota State University,Brookings, United States
*Corresponding author
Abstract:

Endophytic bacteria to promote plant growth by facilitating nutrient acquisition through the fixation of nitrogen, solubilizing phosphate, producing siderophores, producing plant growth hormones, or enzyme1- aminocyclopropane-1- carboxylate (ACC) deaminase and protecting plants from pathogens, via production of antibacterial or antifungal agents, or outcompeting pathogens for nutrients. Isolation and development of new selected plant growth promoting endophytic bacterial strains could be one of the many new approaches that are needed to aid the growth and health of agricultural crops, to eliminate or minimize the harmful effects of inorganic fertilizers, and to conserve organic and inorganic soil nutrients. The aim of this study was to isolate, characterize and identify endophytic bacteria from plants growing along the stream banks in Hot Springs, South Dakota. The bacterial endophytes were isolated, identified and screened in vitrofor morphological features (Gram stain, Gram morphology, and colony morphology). Further, isolates exhibiting difference in morphological features were selected for molecular identification through partial 16S-rRNA gene sequencing. Twenty-five endophytic bacteria strains were isolated from monocotyledons plants, viz. Typha, Bromus tectorum and Festuca and eight strains from a dicotyledonous plant, Nasturtium officinale. All the isolated endophytic bacteria were identified as different bacterial strains belonging to Bacillus thuringensis, B. cereus, B. atrophaeus, Pseudomonas sp., Cedeceadavisae, Escherichia sp., Acinetobacter calcoaceticus, Lysinobacillus sp., Pantoea sp., and Citrobacter freundii. Further investigation is needed to screen these isolated endophytic bacteria for different activities known to promote plant growth and protection from phytopathogen.


Keywords: Endophytic bacteria, Plant growth promotion, Isolation, Characterization, Identification.
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How to cite this article:

Mada Faisal Ashkan and Bruce Bleakley. 2017. Isolation, characterization and identification of putative bacterial endophytes from some plants in hot springs, South Dakota.Int.J.Curr.Microbiol.App.Sci. 6(6): 756-767. doi: https://doi.org/10.20546/ijcmas.2017.606.089