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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 509-520
DOI: https://doi.org/10.20546/ijcmas.2017.606.060


Screening of Microsatellite Markers for Genetic Diversity Assessment and Conservation of Germplasm in Okra (Abelmoschus esculentus L. Moench)
Mukesh Kumar1, V. Rakesh Sharma, Navneet Kumar2, Ujjawal Sirohi3, R.K. Naresh4 and Veena Chaudhary5
1Department of Horticulture,
2Department of Genetics and Plant Breeding,
3College of Biotechnology,
4Department of Agronomy, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, U.P., India
5CSSS (PG) College, Machhra, Meerut-250106, UP, India
*Corresponding author
Abstract:

In the present investigation, an attempt has been made to evaluate the germplasm conservation of some polymorphism information and the genetic diversity in Sixty-nine germplasm of okra collected from the IIVR (59) and IIHR (10), India. Microsatellite marker indicated that a total of 24 distinct bands were found polymorphic with an average of 2.4 bands per primer. The polymorphism information content (PIC) values varied from 0.492 to 0.999 with an average 0.729 and resolving power (RP) varied 0.145 to 3.97 with an average 1.841. Genetic similarity value among the okra germplasm varied from 0.50 to 0.95 with an average 0.772. On the basis of Microsatellite marker, there could be a low level of variation among the germplasm. UPGMA analysis showed that three major groups among the germplasm and dendrogram exhibited that some of the genotypes showed similarity at the molecular level. The results on polymorphism information content, resolving power and microsatellite marker genotypes such as 212-10-1, 167, Kashi Pragati and 1999 were found as diverse parents and promising for using in a breeding programme.


Keywords: Okra, Genetic diversity, Single sequence repeats, Microsatellite markers.
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How to cite this article:

Mukesh Kumar, V. Rakesh Sharma, Navneet Kumar, Ujjawal Sirohi, R.K. Naresh and Veena Chaudhary. 2017. Screening of Microsatellite Markers for Genetic Diversity Assessment and Conservation of Germplasm in Okra (Abelmoschus esculentus L. Moench).Int.J.Curr.Microbiol.App.Sci. 6(6): 509-520. doi: https://doi.org/10.20546/ijcmas.2017.606.060