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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 398-411
DOI: https://doi.org/10.20546/ijcmas.2017.606.047


Banana Bunchy Top Viral Coat Protein (CP) Gene Expression Studies at Molecular Level in Hill Banana cv. Sirumalai (AAB)
Chandrasekar Arumugam*, Kalaiponmani Kalaimughilan and Angappan Kathithachalam
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamilnadu Agricultural University, Coimbatore -641003, India
*Corresponding author
Abstract:

Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. Primers were designed for the PCR amplification of BBTV coat protein (CP). In the present study, to confirm the virus infection and to quantify the viral load in the plants, samples from infected and healthy banana were subjected to PCR and southern blot hybridization. There was no distinguishable difference in viral load with respect to age of the plants was observed. The BBTV capsid protein (CP) gene located on component 3 was cloned in an expression vector pET28a (+). Expression of the CP in E. coli BL21 cells was induced by adding isopropyl-3-D-1-thiogalactoside (IPTG) to a final concentration of 1 mm. The expressed CP which migrated as a protein of approximately 19.5 kDa in Sodium Dodecyl Sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) was identified by its molecular weight. Hence it is concluded that the CP gene of BBTV was cloned and expressed in E. coli can be used for antibody rising or adding an affinity purification tag will enable us to produce pure monoclonal antibody in future.


Keywords: Banana, BBTV, CP, PCR, Cloning, Southern blot, Expression vector.
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How to cite this article:

Chandrasekar Arumugam, Kalaiponmani Kalaimughilan and Angappan Kathithachalam. 2017. Banana Bunchy Top Viral Coat Protein (CP) Gene Expression Studies at Molecular Level in Hill Banana cv. Sirumalai (AAB).Int.J.Curr.Microbiol.App.Sci. 6(6): 398-411. doi: https://doi.org/10.20546/ijcmas.2017.606.047