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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 728-737
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.085


A Lab Originated Bacteriocin and Its Partial Purification and Demonstration of Antimicrobial Activity
Arikta Biswas1 and Rajarshi Banerjee2*
 1Department of Biological Sciences, Indian Institute of Science Education and Research, Mohanpur, Nadia, West Bengal 741246, India
2Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, India
*Corresponding author
Abstract:

Probiotics help maintain human health using different mechanisms. Recently, several reports have been published which deal with such beneficial action which is said to be obtained by the secretion of Lantibiotics. This study focuses on one Probiotic LAB culture isolated from our local niche. This LAB has revealed certain beneficial effects in aquaculture systems. Fourteen potent gastrointestinal pathogens were subjected to the presence of the probable Lantibiotic. Both qualitative and quantitative estimation of the activities was carried out and the antimicrobial activity was expressed in terms of either growth obtained and/or zone of inhibition in different treatments with the LAB originated bacteriocin. The bacteriocin was subjected to several temperature, pH and different salt concentration treatments in order to check its stability and efficacy under such conditions. A number of methods were used to concentrate the extract from the activated LAB. Purification was carried out using TLC as well as by adsorption-desorption method. The results indicate that the bacteriocin can be termed as probable lantibiotic which is quite effective against the gastrointestinal pathogens like Proteus vulgaris and Vibrio parahaemolyticus that have shown to be inhibited by our locally isolated, produced and partially purified LAB originated lantibiotic.


Keywords: Probiotics, Bacteriocins, Antimicrobial activity, Gastrointestinal pathogens.
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How to cite this article:

Arikta Biswas and Rajarshi Banerjee. 2016. A Lab Originated Bacteriocin and Its Partial Purification and Demonstration of Antimicrobial Activity.Int.J.Curr.Microbiol.App.Sci. 5(3): 728-737. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.085