National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Amylase producing bacterial strain, Bacillus cereus MTCC 10205 isolated from soil sample was used for carrying out the present study on optimization of amylase activity. Cultural conditions and nutrients for the maximum production of amylase were optimized by altering one and keeping all other variables as constant. Out of the five media used, Bacillus cereus MTCC 10205 was found to produce maximum amylase (136.70 U) in starch agar medium. The medium contained starch at a concentration of 2% as a sole carbon source and the bacteria was grown for 48 h at 30ºC under submerged fermentation conditions. Optimization of cultural conditions indicated that amylase activity got increased to 294.63 (2.15 fold) when the bacterial strain was cultured at 35ºC for 36 h under static condition with inoculum size 3 per cent (7.5×108 CFU/ml) and using the corn starch as C-source in place of starch and casein hydrolysate as N-source in BOD incubator.
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