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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(6): 136-146
DOI: https://doi.org/10.20546/ijcmas.2017.606.016


Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia
Uma Arumugam*, Nattan Stalin and Gnanadesika Pandian Rebecca
Tamil Nadu Fisheries University, State Referral Laboratory for Fish Disease Diagnosis and Aquatic Animal Health, Fisheries Research and Extension Centre, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India
*Corresponding author
Abstract:

Aquaculture is one of the fastest growing food production sectors globally. Tilapia is the second widely farmed fish species in the global fish production. Enterococcus sp. is one of the leading causes of nosocomial infections in urinary tract, surgical wound and endocarditis in humans. These infections can be hard to treat because of the rising incidence of multiple antibiotic-resistances. The spread of antibiotic resistance has become a major concern in both human and veterinary medicine. In this study, we isolated and characterized an Enterococcus faecalis isolate from a diseased cultured Tilapia. Initial isolation of putative E. faecalis was carried out on streptococcus enrichment broth for 36 h. Characteristic, gram-positive, black color colony was selectively sub-cultured and subsequently identified by 16 rRNA sequencing analysis as Enterococcus faecalis (Genbank Acc no. KT877352). Furthermore, when the isolate was subjected to profiling against 16 antibiotics, it was found to be highly resistant to amoxyclave, ampicillin, erythromycin, gentamicin, kanamycin, nitrofurantoin, penicillin G, streptomycin, sulphafurazole, and vancomycin. The findings of present study showed that E. faecalis infects the cultured Tilapia species and the isolate (SRLFDA/TIL-1/15) possess multiple antibiotic resistance, which emphasizes the urgent need for targeted alternate bio-control strategies for control of emerging diseases like Enterococcus sp. infection in Tilapia culture.


Keywords: Tilapia, Enterococcus faecalis, Multiple-antibiotic resistance, 16s rRNA amplification and sequencing
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How to cite this article:

Uma Arumugam, Nattan Stalin and Gnanadesika pandian Rebecca. 2017. Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia.Int.J.Curr.Microbiol.App.Sci. 6(6): 136-146. doi: https://doi.org/10.20546/ijcmas.2017.606.016