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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2882-2892
DOI: https://doi.org/10.20546/ijcmas.2017.605.328


Utilization of molecular and morphometric tools for assessment of genetic diversity of Rice bean [Vigna umbellata (Thunb.) Ohwi and Ohashi]
V. S. Meena1*, R. K. Mittal2, P. R. Choudhury1, S. Rathod3, H. K. Mahadevaswamy4 and R. Choudhary5
1Crop Science Division, ICAR Headquarters, Krishi Bhawan, New Delhi -1100 01, India
2Department of Crop Improvement, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur -176062, India
3ICAR-Indian Agricultural Statistics Research Institute, New Delhi – 1100 12, India
4ICAR-Sugarcane Breeding Institute, Coimbatore – 6410 07, Tamil Nadu, India
5Gobind Ballabh Pant University of Agriculture & Technology, Pantnagar, 2631 53, India
*Corresponding author
Abstract:

Rice bean [Vigna umbellata (Thunb.) Ohwi and Ohashi] is an orphan grain legume, grown in several parts of the country and has a pivotal role as a pulse in supporting the food security of the rural poor people, particularly in hill areas. Multipurpose uses and high nutritional quality of the crop gains its importance in the future food security needs of the country. Despite many useful characteristics, it has not been subjected to systematic breeding, hence a study was undertaken to study the diversity of the available germplasm collected from Himachal Pradesh. In the present study, RAPD markers were employed to study the genetic diversity, a RAPD profile of 64 rice bean genotypes generated 83 bands, of which 47 were polymorphic (56.62% of polymorphism) with Jacard’s similarity coefficient matrices variation from 0.48 to 1.00. Based on the polymorphism exhibited by RAPD markers, 64 rice bean genotypes were grouped into 2 main clusters which again sub divided into several sub-clusters. PCA analysis gave the 12 principal components; among them first two were accounted for 61.65% of the total variability. A biplot plotted, showed a positive correlation among all the traits except crude protein with days to fifty percent flowering and days to fifty percent maturity. Results revealed from the study were preliminary and can be utilized in rice bean improvement programmes and further advanced studies.


Keywords: Rice bean, Diversity, Vigna, RAPD, Morphometric
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How to cite this article:

Meena V. S., R. K. Mittal, P. R. Choudhury, S. Rathod, H. K. Mahadevaswamy and Choudhary R. 2017. Utilization of molecular and morphometric tools for assessment of genetic diversity of Rice bean [Vigna umbellata (Thunb.) Ohwi and Ohashi].Int.J.Curr.Microbiol.App.Sci. 6(5): 2882-2892. doi: https://doi.org/10.20546/ijcmas.2017.605.328