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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 667-675
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.078


Prevalence of biofilm production by Staphylococcus species isolated from patients on indwelling medical devices/implants
Priyanka Khanna1*, Pushpa Devi2 and Bimla Devi2
1Department of Microbiology, PIMS Medical College, Jalandhar, Punjab, India
2Department of Microbiology, Government Medical College, Amritsar, Punjab, India
*Corresponding author
Abstract:

The increased use of indwelling medical devices has had considerable impact on the role of Staphylococci in clinical medicine, their major pathogenic factor being ability to form biofilm on polymeric surfaces. Bacteria growing within a biofilm lose their sensitivity to antibiotics quickly. Thus biofilms result in persistent infections that cannot be resolved with standard antibiotic treatments. The present study was done to know the ability for biofilm production and the antibiotic susceptibility among different species identified from 250 clinicalisolates of Staphylococci. Three standard methods of Tissue culture plate, Tube method and Congo Red Agar method were used for detection of biofilm production. Biofilm production was detected in 172 (68.80%) of 250 staphylococcal isolates. Staphylococcus aureus accounted for 88 (51.16%) and S. epidermidisfor 65(37.79%) followed by S. haemolyticus which accounted for 19 (11.05%) biofilm producing Staphylococcal isolates. Persistent infection due to biofilm formation is certainly a new and additional burden to clinicians. Identification of biofilm production and its relation with antimicrobial resistance might help to elucidate the impact of Staphylococci in diagnosis of various infections.


Keywords: Biofilm, Tissue Culture plate, Congo red agar, Indwelling medical devices, Staphylococcus.
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How to cite this article:

Priyanka Khanna, Pushpa Devi and Bimla Devi. 2016. Prevalence of biofilm production by Staphylococcus species isolated from patients on indwelling medical devices/implants.Int.J.Curr.Microbiol.App.Sci. 5(3): 667-675. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.078