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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2714-2721
DOI: https://doi.org/10.20546/ijcmas.2017.605.304


Microsatellite in Coriander: A Cross Species Amplification within Apiaceae Family
Sharda Choudhary1*, Geetika Jethra2, Radheshyam Sharma1 and Arvind Kumar Verma1
1ICAR-National Research Centre on Seed Spices, Ajmer, Rajasthan-305 206, India
2Banasthali Vidyapith, P.O. Banasthali Vidyapith, Rajasthan-304022, India
*Corresponding author
Abstract:

Coriander is a spice crop mainly used for seasoning in cooking. For the improvement of this crop very limited literature is available for molecular studies. Hence, it’s a need to develop molecular markers for this crop. Development of SSRs (Simple Sequence Repeats) for new crop like coriander is an expensive and time consuming process. This can be easily achieved by transferring the microsatellile loci from the same family/genus/species which is a cost-effective approach for development of SSRs in lesser studied crops like coriander. Presently in carrot and celery SSRs are available on the public domain for cross-species investigation, both crops belongs to the same family. We examined the transferability of hundred carrot SSR loci to coriander. Thirty nine percent primer (39/100) amplified SSRs for coriander. Out of 39 SSR loci, 35 primers were monomorphic and specific to carrot primers, showing approximately the same size of SSR loci as reported for carrot. Four SSRs were polymorphic, seven primer pairs were non-specific which were deviating for band size from the source of SSRs. Primer GSSR-113 was non-specific, but showing polymorphism for the microsatellite loci. These SSR markers should provide a powerful tool for coriander breeding and genetics.


Keywords: Apiaceae, Coriandrum sativum, Cross-Transferability, Microsatellite, SSRs
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How to cite this article:

Sharda Choudhary, Geetika Jethra, Radheshyam Sharma and Arvind Kumar Verma. 2017. Microsatellite in Coriander: A Cross Species Amplification within Apiaceae Family.Int.J.Curr.Microbiol.App.Sci. 6(5): 2714-2721. doi: https://doi.org/10.20546/ijcmas.2017.605.304