Metabolomics approaches enable the parallel assessment of the levels of a broad range of metabolites and have been great value in both phenotyping and diagnostic analyses in plants and other organisms. Analysis of metabolites was done by using different method like HPLC, GC, CE, MS etc but due to problem in their efficiency, accuracy and speed they are replaced by new throughput instrument like GC-MS, HPLC-MS, CE-MS, NMR, GC-MS-TOF etc. These high through instrument gives better result over traditional one in respect to their efficiency, accuracy and speed for the analysis of huge number of metabolite in the cell. Metabolomic approach nowadays effectively used to identify mQTL in plant which benefical in breeding programme for the selection of progeny in short period of time that of MAS selection. Metabololomics not only used in breeding programme but also make effective role in diagnosis and prediction of different disease in human. Comprehensive study of human metabolites “human metabolome project” was lunched jan-2005 in Canada. Metabolomics technologies are needed to cover the wide array of phytochemicals and increase the spatial and temporal resolution of metabolome analysis. Strengthening of spectral databases and sophisticated informatics for more accurate annotation of metabolites is necessary, and these efforts must be internationally harmonized and publicly available.

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