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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 643-650
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.075


Detection of biofilm formation among the clinical isolates of Enterococci: An evaluation of three different screening methods
L.Triveda* and S. Gomathi
Department of microbiology SRM Medical College Hospital and Research Centre Kattankulathur, Tamilnadu, India
*Corresponding author
Abstract:

This study was conducted to evaluate three methods for the detection of biofilm formation in Enterococci. For the detection of biofilm production ,128 clinical isolates of Enterococcus spp were screened by tissue culture plate (TCP), Tube method (TM)  and Congo red agar (CRA) method. Of the 128 Enterococcus spp, 71( 55.5%) isolates showed a biofilm positive phenotype under the optimized conditions in the TCP method and strains were further classified as strong 28 (31.9%) ,moderate 22 (17.2%) ,weak 21 (16.5%) wherein 57(44.6 %) isolates no biofilm was detected. Though TM correlated well with the TCP method for 14 (11%) strongly biofilm producing strains, weak producers were difficult to discriminate from biofilm negative isolates We can conclude from our study that the TCP method is a reliable and sensitive screening method for the detection of biofilm formation by Enterococci when compared to TM and CRA methods.TCP methodalso has the advantage of being a quantitative model to study the adherence of Enterococci and  can be recommended as a general screening method for detection of biofilm producing Enterococci in laboratories.


Keywords: Enterococci, Adherence , Biofilms , Tissue Culture Plate, Congo Red Agar, Tube Method.
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How to cite this article:

Triveda, L., and S. Gomathi. 2016. Detection of biofilm formation among the clinical isolates of Enterococci: An evaluation of three different screening methods.Int.J.Curr.Microbiol.App.Sci. 5(3): 643-650. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.075