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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2399-2408
DOI: https://doi.org/10.20546/ijcmas.2017.605.268


Draft Genome Sequence of a Haloalkaliphilic archaeon: Natrialba sp. SSL1 (ATCC 43988) Isolated from Sambhar Salt Lake, India
G.N. Kalambe1, P.M. Chandarana2, V.M. Tanavade2,3,4 and V.N. Upasani5*
1JJTU, Vidyanagari, Jhunjhunu-333001, Rajasthan, India 2IBioAnalysis Pvt. Ltd., Ahmedabad 380009, Gujarat, India 3Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Ahmedabad 380009, Gujarat, India 4Bioinformatics Institute, Agency for Science Technology and Research (A*STAR), 30 Biopolis Street, Singapore 138671 5Department of Microbiology, M. G. Science Institute, Ahmedabad 380009, Gujarat, India
*Corresponding author
Abstract:

An extremely haloalkaliphilic archaeon, Natrialba sp. SSL1, gram-negative, rod-shaped, motile, aerobic, chemoorganotrophic belonging to the family Halobacteriaceae within the Phylum Euryarchaeota was isolated from Sambhar Salt Lake (SSL), Rajasthan, India in the 1980s. The Whole Genome Sequence (WGS) of this archaeon was deciphered for the purpose of comparative genomics with other halobacteria as well as eubacteria. The WGS raw data of the genome was assembled into 61 contigs, showing total sequence length of 4,580,837bp, comprising of 4276 genes, out of which 4126 were found to be coding genes (exons), while 96 were psuedogenes. It encodes for 4048 proteins, some of which are peptide repeats of various lengths. Comparative genomic analysis facilitated the identification of genes encoding proteins involved in glycosylation, synthesis of novel archaeal isoprenoid glycolipid identified as glucopyranosyl-1, 6-glucopyranosyl-1-glycerol diether (DGD-4), bacteriocin (halocin), adaptation to salinity stress response, etc. Based on genomic analysis, Natrialba sp. SSL1is metabolically versatile and can grow on various carbon and nitrogen sources. Presence of photosystem reaction centre subunit H indicates parallel photosynthetic proton generation system. Genes annotation revealed the presence of extremozymes like alpha-amylase, lipase, protease, trehalose phosphatase, etc. that can be exploited further for biotechnological purpose. This is the first haloalkaliphilic archaeal genome sequenced from India.


Keywords: Natrialba SSL1, haloalkaliphiles, haloarchaea, Whole Genome Sequencing (WGS), IlluminaHiseq, soda lakes, Sambhar Lake
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How to cite this article:

Kalambe, G.N., P.M. Chandarana, V.M. Tanavade and Upasani, V.N. 2017. Draft Genome Sequence of a Haloalkaliphilic archaeon: Natrialba sp. SSL1 (ATCC 43988) Isolated from Sambhar Salt Lake, India.Int.J.Curr.Microbiol.App.Sci. 6(5): 2399-2408. doi: https://doi.org/10.20546/ijcmas.2017.605.268