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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 624-634
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.073


Molecular characterization of α-amylase producing Brevibacillus borstelensis R1 isolated from Bay of Bengal, Visakhapatnam, India
K.Suribabu1* and K.P.J Hemalatha2
1Department Microbiology and Research Centre, Dr.Lankapalli Bullayya College, Visakhapatnam-530 013.A.P, India
2Department Microbiology, Andhra University, Visakhapatnam-530 003.A.P, India
*Corresponding author
Abstract:

The present study is aimed to screen the potent α-amylase producing bacteria from the waters of Bay of Bengal, Visakhapatnam. As Bacillus species proved efficient in many industrial applications, our study is mainly focused on molecular characterization of potent α-amylase producing Bacillus species.  The water samples were collected along the coast of Bay of Bengal, at four sites- Rushikonda, Appughur, Fishing harbor, Gangavaram and were cultured on enriched starch agar medium. The bacteria screened during the study (Aug., 2008 - Jun., 2010) were of Bacillus species. Out of 1618 isolates, 143 isolates exhibited 3-11mm clear zone of starch hydrolysis, 17 exhibited above 11mm and the rest of them yielded less than 3mm zone. By determining the α-amylase production of the isolates by 3, 5-Dinitro salicylic acid method, the high yielding 17 isolates were selected for identification. By using protocols from Bergey’s Manual Determinative Bacteriology, the colony, morphological and biochemical characterization of the high yielding α-amylase bacteria were identified and classified into five groups. The highest α-amylase yielding isolate was identified as Brevibacillus borstelensis R1 through 16S rRNA gene sequence analysis and Mega BLAST score. BLASTN score was employed to construct the phylogenetic tree. The enzyme ability in starch processing, textile industry, improving shelf life of bread, ethanol production, sewage treatment and effluent treatment of industries are studied.


Keywords: Mega BLASTN score; Brevibacillus borstelensis R1; phylogenetic tree; 16S rRNA gene sequence analysis.
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How to cite this article:

Suribabu, K., and K.P.J Hemalatha. 2016. Molecular characterization of α-amylase producing Brevibacillus borstelensis R1 isolated from Bay of Bengal, Visakhapatnam, IndiaInt.J.Curr.Microbiol.App.Sci. 5(3): 624-634. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.073