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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2264-2270
DOI: https://doi.org/10.20546/ijcmas.2017.605.252


Molecular Detection and Subtyping of New Delhi Metallobetalactamases (NDM-1) in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in a Tertiary Care Hospital, South India
M. Uma Maheswari* and Appalaraju
Department of Microbiology, PSGIMS & R, Coimbatore, India
*Corresponding author
Abstract:

Enterobacteriaceae are inhabitants of human, animal gastrointestinal tract and various other environmental sites. Escherichia coli and Klebsiella pneumoniae initially responded to betalactam antibiotics but there was an increase in resistance to betalactam antibiotics due to beta lactamase produced by them. Resistance to carbapenems has now become more prevalent due to production of carbapenemases. NDM-1 is a novel metallo betalactamase that is capable of hydrolysing all beta lactam antibiotics except Aztreonam. It was aimed for Molecular detection and subtyping of NDM-1 in Escherichia coli and Klebsiella pneumoniae. Hundred carbapenem resistant isolates (42 isolates of Escherichia coli and 58 isolates of Klebsiella pneumoniae) were selected randomly for Molecular detection and sequencing of subtypes. A total of 82% strains were found to be NDM-1 positive yielding 475bp amplicon by molecular methods. Nine samples were sequenced for subtypes, of which five were NDM-1, two were NDM-4, and remaining two were NDM-3, and NDM-5.Sequencing and finding out of the subtypes may help to select new drug targets and design novel antibiotics.


Keywords: NDM-1- Newdelhi, metallo-betalactamases
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How to cite this article:

Uma Maheswari, M. and Appalaraju. 2017. Molecular Detection and Subtyping of Newdelhi Metallobetalactamases (NDM-1) in Clinical Isolates of  Escherichia coli and Klebsiella pneumoniae in a Tertiary Care Hospital, South India.Int.J.Curr.Microbiol.App.Sci. 6(5): 2264-2270. doi: https://doi.org/10.20546/ijcmas.2017.605.252