National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Enterobacteriaceae are inhabitants of human, animal gastrointestinal tract and various other environmental sites. Escherichia coli and Klebsiella pneumoniae initially responded to betalactam antibiotics but there was an increase in resistance to betalactam antibiotics due to beta lactamase produced by them. Resistance to carbapenems has now become more prevalent due to production of carbapenemases. NDM-1 is a novel metallo betalactamase that is capable of hydrolysing all beta lactam antibiotics except Aztreonam. It was aimed for Molecular detection and subtyping of NDM-1 in Escherichia coli and Klebsiella pneumoniae. Hundred carbapenem resistant isolates (42 isolates of Escherichia coli and 58 isolates of Klebsiella pneumoniae) were selected randomly for Molecular detection and sequencing of subtypes. A total of 82% strains were found to be NDM-1 positive yielding 475bp amplicon by molecular methods. Nine samples were sequenced for subtypes, of which five were NDM-1, two were NDM-4, and remaining two were NDM-3, and NDM-5.Sequencing and finding out of the subtypes may help to select new drug targets and design novel antibiotics.
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