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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2090-2099
DOI: https://doi.org/10.20546/ijcmas.2017.605.233


Comparative Sensitivity and Specificity of Various Serological Tests for Detection of Brucellosis in Small Ruminants
R.R. Padher*, J.B. Nayak, M.N. Brahmbhatt and R.A. Mathakiya
Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
*Corresponding author
Abstract:

Brucellosis is a zoonosis known to be a major public health hazard of great economic importance globally. The present study was taken up to ascertain the seroprevalence of brucellosis in goats, sheep, and humans of Anand, districts of the Central Gujarat region. A total of 200 serum samples were collected from the goat and sheep of Anand district and subjected to different serological test i.e., Rose Bengal Plate test (RBPT), Standard Tube Agglutination Test (STAT) and Indirect ELISA to detect the Brucella antibody and also compared in terms of their sensitivity and specificity. A total 200 sera sample including 100 from goats and 100 from sheep collected from the Anand district. Among 200 sera samples of sheep and goats, 94 (47.00%) samples were found to be positive. 93 sera samples were positive by I-ELISA, 27 by RBPT while 16 by STAT. One RBPT positive goat serum sample was found to be negative by I-ELISA. Fifty six goat sera samples were found to be sero-positive, out of which 55 samples were found to be positive by I-ELISA while 16 by RBPT and 10 by STAT. Among 38 sera samples of sheep found to be positive by I-ELISA while 11 by RBPT and 6 by STAT. In case of goats, the sensitivity of RBPT and STAT was 27.27 and 18.18 per cent while specificity was 97.82 and 100 per cent, respectively. An overall agreement of RBPT and STAT with I-ELISA was 66.00 and 55.00 per cent, respectively. Thus, sensitivity of RBPT was more than STAT but specificity of RBPT and STAT was somewhat similar. In case of sheep, the sensitivity of RBPT and STAT was 28.95 and 15.78 per cent while specificity was 100 per cent for both test. An overall agreement of RBPT and STAT with I-ELISA was 73.00 and 68.00 per cent, respectively. Thus, sensitivity of RBPT was more than STAT but specificity of RBPT and STAT was similar.


Keywords: Brucellosis, Specificity, Sensitivity, Small ruminants
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How to cite this article:

Padher, R.R., J.B. Nayak, M.N. Brahmbhatt and Mathakiya, R.A. 2017. Comparative Sensitivity and Specificity of VariousSerological Tests for Detection of Brucellosis in Small Ruminants.Int.J.Curr.Microbiol.App.Sci. 6(5): 2090-2099. doi: https://doi.org/10.20546/ijcmas.2017.605.233