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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2067-2071
DOI: https://doi.org/10.20546/ijcmas.2017.605.230


Molecular Identification of Lipase Producing Bacteria based on 16S rDNA Sequencing
P. Pallavi*, P. Bhavani, J. Komali and T. Manjusha
Department of Microbiology, Government Degree College for Women, Nalgonda, India
*Corresponding author
Abstract:

Lipases or triacyl glycerol acylester hydrolases or carboxyl esterases (E.C 3.1.1.3) that catalyze both hydrolysis and synthesis of esters formed from glycerol. Lipases are currently attracting an enormous attention because of their biotechnological applications. In particular, lipases of microbial origin finding immense applications in various fields as they can catalyze a variety of hydrolytic or synthetic reactions. A bacterial strain isolated from an oil contaminated soil using Nutrient agar medium with 1% olive oil. The isolated strains were screened for lipolytic activity on tributyrin agar and the lipolytic potential was measured. The strains with lipolytic potential (R/r) >2 were selected and further screened for lipase production on ideal medium. The Lipase assay was carried out by measuring the growth using optical density at regular time intervals of 24hrs, 48hrs and 72hrs respectively. The selected bacterial strain with maximum lipase production was observed at 48hrs, 37oC (9.0 EU/ml). In our studies, the best producer of lipase was subjected to molecular identification based on 16S r DNA nucleiotide sequence homology and phylogenetic analysis, a newly isolated indigenous potential lipase producing strain (LP5) was identified as Bacillus subtilis strain Y-IVI.


Keywords: Molecular, Lipases, Deacetylation, Isolation
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How to cite this article:

Pallavi, P., P. Bhavani, J. Komali and Manjusha, T. 2017. Molecular Identification of Lipase Producing Bacteria based on 16S rDNA SequencingInt.J.Curr.Microbiol.App.Sci. 6(5): 2067-2071. doi: https://doi.org/10.20546/ijcmas.2017.605.230