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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1992-2004
DOI: https://doi.org/10.20546/ijcmas.2017.605.223


Isolation and Characterization of Nodule Associated Bacteria from Chickpea and their Potential for Plant Growth Promotion
Deepak Kumar Koli* and Karivaradharajan Swarnalakshmi
Division of Microbiology, ICAR-Indian Agricultural Research Institute (IARI), New Delhi 110012, India
*Corresponding author
Abstract:

A total of seventy three NAB were isolated from surface sterilized nodules of different chickpea (Cicer arietinum) cultivars grown in the field from IARI, New Delhi using various kinds of media. Screening for the improved seedling growth showed that 91.78% of them enhanced radical length, and 87.67% of them increased plumule length under in-vitro conditions. About twenty four isolates were selected from preliminary screening and were subjected to their growth promoting potential under pot culture conditions using selected cultivar (PUSA 372). The promising nodule associated bacteria (NAB 69) showed a significant increase in plant growth regarding shoot dry weight (40.63%) and root dry weight (45.09%) in comparison to control. Its interactive effect was evaluated with Mesorhizobium ciceri for growth attributes and nodulation potential under pot culture conditions. The highest nitrogenase activity (74.12 μmoles C2H4/g of fresh weight of nodules/hr) was recorded in the treatment involving NAB 69 along with Mesorhizobium ciceri at 45 DAS (Vegetative stage). In-vitro screening for the functional potential of NAB showed a positive result with P solubilization. However, nitrogenase activity and siderophore production were not detected in the selected bacterial isolate. The 16S rDNA sequencing revealed that the bacterial isolate was closely related to Enterobacter sp. with 99% maximum identity. This investigation led to identifying the natural associations between the most promising nodule associated bacterium and Mesorhizobium ciceri in chickpea and the positive influence of their interactions.


Keywords: Chickpea, Endophytes, Root nodules, Growth promoting traits, Rhizobium, Nitrogen fixation, 16S rRNA
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How to cite this article:

Deepak Kumar Koli and Karivaradharajan Swarnalakshmi. 2017. Isolation and Characterization of Nodule Associated Bacteria from Chickpea and their Potential for Plant Growth PromotionInt.J.Curr.Microbiol.App.Sci. 6(5): 1992-2004. doi: https://doi.org/10.20546/ijcmas.2017.605.223