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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1872-1876
DOI: https://doi.org/10.20546/ijcmas.2017.605.206


Necrotic Enteritis by Beta2toxin-Producing Clostridium perfringens in Doom Pigs of Assam, India
Md. Iftikar Hussain1,4*, Probodh Borah3,4, IsfaqulHussain6, Rajeev Kumar Sharma5 and Mohan Chandra Kalita2
1Department of Bioengineering and Technology,
 2Department of Biotechnology, Gauhati University, Assam, India
3Department of Animal Biotechnology,
 4State Biotech Hub,
5Department of Veterinary Microbiology, College of Veterinary Science, AAU, Assam, India
6Division of Veterinary Microbiology and Immunology, FVSc and AH,SKUAST-Kashmir, J&K, India
*Corresponding author
Abstract:

Piglet diarrhoea caused by beta2 toxin producing Clostridium perfringens is a serious problem to piggeries throughout the world. The newly recognized Doom breed of Assam, India can survive under poor rearing condition and is generally found to be disease resistant. Beta2 toxin was earlier reported to be associated with necrotic enteritis in piglets of different breeds but its role has not so far been reported in Doom breed. Two cases of piglet diarrhoea in their post mortem analysis were found to be havingsevere lesions of necrotic enteritis in their intestine. Bacterial isolation followed by biochemical and molecular analysis confirmed theirassociation with C. perfringens Type A possessing beta2 gene. Further, to findout the total C. perfringens load under such severe condition, it was quantified by qPCR anda maximum of 2 x 106 fold incerease was observed compared to thereported load for healthy piglets.


Keywords: Doom pig, Nectrotic enteritis, qPCR, Multiplex, Beta2
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How to cite this article:

Md. Iftikar Hussain, Probodh Borah, IsfaqulHussain, Rajeev Kumar Sharma and Mohan Chandra Kalita. 2017. Necrotic Enteritis by Beta2toxin-Producing Clostridium perfringens in Doom Pigs of Assam, IndiaInt.J.Curr.Microbiol.App.Sci. 6(5): 1872-1876. doi: https://doi.org/10.20546/ijcmas.2017.605.206