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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2246-2258
DOI: https://doi.org/10.20546/ijcmas.2017.605.250


Variations in Stress Tolerance Abilities of Diverse Listeria monocytogenes Isolates
Satyajit B. Kale1,4, Nitin V. Kurkure1, Swapnil P. Doijad2, Krupali V. Poharkar3,Sandeep Garg4, Deepak B. Rawool5 and Sukhadeo B. Barbuddhe3*
1Department of Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India
2Institute of Medical Microbiology, Justus-Liebig University, 35392 Giessen, Germany
3ICAR-National Institute of Biotic Stress Management, Baronda, Raipur, 493225, India
4Department of Microbiology, Goa University, Taleigaon Plateau, Goa 403 206, India
5Division of Veterinary Public Health, Indian Veterinary Research Institute,Izatnagar, 243122, India
*Corresponding author
Abstract:

Listeria monocytogenes is an important foodborne pathogen with the ability to survive and grow in different foods and food processing environments. The variability in innate stress tolerance abilities of L. monocytogenes strains (n=104) isolated from clinical (n=35), environment (n=28) and food (n=41) sources was investigated against salt (2.5% to 12.5%), pH (pH 4.0 to 9.5) and low temperature (down to 4⁰C). The stress tolerance abilities were correlated with the source of isolation, serogroups and identifying the prevalent stress tolerant genotype. A total of 37 (35.57%) strains could tolerate different stresses of which 19 (18.26%) strains showed multi-stress tolerance capability. No correlation was observed among tolerance pattern and sources of isolation, while, 46.55% strains of L. monocytogenes serogroup 4b, 4d, 4e were tolerant to different stresses. The subtyping of stress tolerant strains employing pulsed-field gel electrophoresis revealed 15 pulsotypes. Multiple stress tolerant strains belonging to serogroup 4b, 4d, 4e (n= 21) revealed to be clonal with unique pulsotypes. However, no correlation was observed for particular stress and pulsotypes. The data showed that strains varied remarkably with respect to stress tolerance abilities under different stresses without any correlation between stress tolerance pattern and origin of the strains for all studied stresses. This study is a significant step towards dissecting the variability of stress response in L. monocytogenes and understanding the dominance and prevalence of particular serogroup among different niches.


Keywords: Listeria monocytogenes, Serogroups, Stress tolerance, pH, Salt, Low temperature
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How to cite this article:

Satyajit B. Kale, Nitin V. Kurkure, Swapnil P. Doijad, Krupali V. Poharkar, Sandeep Garg, Deepak B. Rawool and Sukhadeo B. Barbuddhe. 2017. Variations in Stress Tolerance Abilities of Diverse Listeria monocytogenes Isolates.Int.J.Curr.Microbiol.App.Sci. 6(5): 2246-2258. doi: https://doi.org/10.20546/ijcmas.2017.605.250