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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2209-2219
DOI: https://doi.org/10.20546/ijcmas.2017.605.247


An Estimation of Effective Mutagen Doses, Induced Genetic Variation and Characters Association in M2 Generation of Okra
Rajkumari Ashadevi1, A.V.V. Koundinya2* and S.B. Chattopadhyay1
1Department of Vegetable Crops, Bidhan Chandra Krishi Viswavidyalaya,Mohanpur, Nadia, West Bengal, India
2Division of Crop Improvement, ICAR-CTCRI, Sreekaryam, Trivandrum, Kerala, India
*Corresponding author
Abstract:

The experiment was done with two mutagenic agents viz., Gamma radiation (γ) and EMS and two varieties namely BCO-1 and Japanese Jhar Bhendi. Seeds were irradiated with gamma rays at 100, 200, 300, 400, 500 kRγ and treated with 0.1, 0.2, 0.3, 0.4 and 0.5 per cent EMS concentration. A total of 10gamma irradiated lines and 10 EMS treated lines along with dry and wet controls of the two varieties were grown in M2 generation. The first two principle components having Eigen values more than one were cumulatively contributing 99.57% to the total induced variation. The PC-1 associated with 300 kR γ, 400 kR γ, 500 kR γ, 0.3% EMS, 0.4% EMS and 0.5% EMS was contributing highly 52.86% to the variation. Both the mutagens (γ and EMS) induced more variation at higher doses than at their lower doses. Dendrogram explained that the mutagenic treatments could be distinguished into four different groups when less than 5% variation was considered.The treatments 100kR γ and 0.2% EMS were found to be highly diverse from the rest of the treatments. Both gamma irradiation and soaking in EMS caused sufficiently high GCV and PCV for effective selection to carry out in the characters as the first node to fruit, the number of flowers per plant, the number of fruits per plant and the number of seeds per pod and seed index. High heritability coupled with high GAM was observed for days to first flower set, first node to flower, first node to fruit, number of flowers per plant, number of fruits per plant, number of seeds per pod, seed index and fruit yield per plant in both the populations and for number of primary branches/plant when treated with gamma radiation; for fruit length when soaked in EMS suggesting that these characters were under strong influence of additive gene action. Positive association of fruit yield per plant with the number of flowers per plant and number of fruits per plant and negative association with days to first flower set and first fruiting node in both populations suggesting importance of these characters while selecting high yielding genotypes.


Keywords: Mutation,Okra, Comparison of mutagens, Genetic variation and characters association
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How to cite this article:

Rajkumari Ashadevi, A.V.V. Koundinya and Chattopadhyay, S.B. 2017. An Estimation of Effective Mutagen Doses, Induced Genetic Variation and Characters Association in M2 Generation of Okra.Int.J.Curr.Microbiol.App.Sci. 6(5): 2209-2219. doi: https://doi.org/10.20546/ijcmas.2017.605.247