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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2186-2208
DOI: https://doi.org/10.20546/ijcmas.2017.605.246


Isolation and Characterization of Phyllospheric Methylotrophic Bacteria of Paddy from Middle Gujarat, India
Ronak R. Prajapati*, R.V. Vyas and Y.K. Jhala 
Department of Agricultural Microbiology and Biofertilizers Project, B. A. Collage of Agriculture, Anand Agricultural University, Anand-388110, Gujarat, India
*Corresponding author
Abstract:

Phyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Among 33 isolates three potent isolates viz. M 3, M 10 and M 15 showed fourfold increase in cell numbers in methanol 1% solution. Isolate M 3, M 10 and M 15 were found Gram +ve, rods occurring singly. Isolate M 3, M 10 and M 15 showed yellow, dull white and shiny white pigmented growth respectively, on NMS media. From cultural and microscopic characteristics it was accepted that, isolates belongs to family Bacillaceae. Isolate M 15 were found tolerating higher pH range (9.0) and 7.5% NaCl concentration as compared to other isolates Isolate M 10 and M 15 showed resistant to wide range of antibiotics and were found to utilize carbon sources. On the basis of partial 16S rRNA gene sequencing selected phyllospheric isolates, AAU M 3 was identified as Staphylococcus saprophyticus, AAU M 10 as Bacillus subtilis, AAU M 15 as Bacillus methylotrophicus. Chosen three isolates were subjected to qualitative detection of two key enzymes of bacterial methane degradation pathway viz. soluble methane monooxygenase (sMMO) and methanol dehydrogenase (MDH) and established positive.


Keywords: Phyllosphere, Methylotrophs, Soluble methane monooxygenase, Methanol dehydrogenate, Paddy
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How to cite this article:

Ronak R. Prajapati, R.V. Vyas and Jhala, Y.K. 2017. Isolation and Characterization of Phyllospheric Methylotrophic Bacteria of Paddy from Middle Gujarat.Int.J.Curr.Microbiol.App.Sci. 6(5): 2186-2208. doi: https://doi.org/10.20546/ijcmas.2017.605.246