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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 579-589
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.067


A Comparative Analysis on Identification and Antifungal Susceptibility Profile of Candida Isolates Using Conventional and Automated Techniques
S. Prasanna1, Partha Roy2, Naveen Grover3, Puneet Bhatt4, Dudhat Vaibhav Laxmikant Rao5 and Nikunja Kumar Das6
1Department of Microbiology, Shri Sathya Sai Medical College and Research Institute, Ammapettai, Kancheepuram-608103, India
2Department of Microbiology, Delhi Base Hospital, Delhi, India
3Department of Microbiology, AFMC, Pune-411040, India
4Department of Microbiology, CH (SC), Pune-411040, India
5Consultant Microbiologist, Sayadhri Hospital, Pune, India
6Department of Microbiology, D.Y.Patil Medical College, Pune, India
*Corresponding author
Abstract:

Candida species are the most common fungal pathogen isolated from clinical samples. Of late, improper use of antifungal drugs in critically ill patients has favoured antifungal drug resistance amongst Candida. To isolate and identify Candida species from hospitalized patients and determine their antifungal susceptibility profile by conventional (Epsilometer i.e., E-test) and automated (VITEK-2) methods. This cross sectional study was carried out from December 2012 to June 2014 from hospitalized patients at a tertiary care centre, in which 50 non-repetitive clinical isolates of Candida were analysed. The identification was carried by conventional methods as per standard laboratory protocols and by VITEK-2 Compact (Biomerieux, France). The antifungal susceptibility testing (AFST) of these isolates was carried out by E-test and VITEK-2 Compact (YST cards- AST YS01). Out of 50 isolates, 36 (72%) were non-albicans Candida and 14 (28%) were C.albicans. AFST by VITEK-2 showed that only 2 isolates (4%) were resistant to fluconazole and E-test showed that 24 isolates (48%) were resistant to fluconazole and voriconazole but all were sensitive to amphotericin B. There was substantial agreement of 80% (95% CI of 69 – 91%) for identification and less than chance agreement for AFST between VITEK-2 and E-test.  We conclude that E-test can be a simple and convenient method for AFST when compared to VITEK-2. We cannot rely only on VITEK-2 for AFST. Identification and AFST of Candida isolates must be carried out for optimum patient care.


Keywords: Candida species, Antifungal susceptibility testing, VITEK-2, E-test.
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How to cite this article:

Prasanna, S., Partha Roy, Naveen Grover, Puneet Bhatt, Dudhat Vaibhav Laxmikant Rao and Nikunja Kumar Das. 2016. A Comparative Analysis on Identification and Antifungal Susceptibility Profile of Candida Isolates Using Conventional and Automated TechniquesInt.J.Curr.Microbiol.App.Sci. 5(3): 579-589. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.067