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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1662-1676
DOI: https://doi.org/10.20546/ijcmas.2017.605.181


Evaluation of Streptomyces Common Scab Toxins Diffusion in Potato Tubers and through the Intestinal Barrier
Camille Leclerc1, Arthur Zebré1, Jérémy Enault1, Pierre-Jean Racine1, Sandra Leblanc2, Nathalie Connil1, Pascal Svinareff2 and Marc G.J. Feuilloley1*
1Laboratory of Microbiology Signals and Microenvironment LMSM, EA 4312,University of Rouen, Normandie, F-27000 Evreux, France
2Biogalenys SAS, 9 rue de Pacy, F-27930 Miserey, France
*Corresponding author
Abstract:

Streptomyces and particularly Streptomyces scabies the causative agent of common scab in potato tubers are producing different toxins, including thaxtomin A, nigericin, geldanamycin, tuncamycin and bafilomycin. Assay of these toxins in two potato strains from the commercial circuit revealed that thaxtomin A can be detected in significant amounts in scab surface lesions but also in the flesh of the tubers. Other toxins were present under the detection limit of the assay. As thaxtomin A and other Streptomyces toxins are thermostable, potato consumers can be exposed to these molecules. In order to evaluate the potential effect of Streptomyces toxins on the intestinal barrier, their cytotoxicity was studied in-vitro on Caco2/TC7 enterocytes. Except geldanamycin, all other tested toxins showed significant cytotoxicity. Used at sub-lethal concentrations, Streptomyces toxins were without effect on the transepithelial resistance of a model of intestinal epithelium made by fully differentiated Caco2/TC7 cells cultured in inserts. However, measurements of the flow of these toxins through reconstructed intestinal epithelium suggest that thaxtomin A, geldanamycin and tuncamycin could cross the intestinal barrier. These results should deserve in-vivo studies to evaluate the real threat of these toxins in regard of consumers.


Keywords: Potatos, Streptomyces scabies, Thaxtomin A, Nigericin, Bafilomycin A1, Caco2/TC7 cells.
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How to cite this article:

Camille Leclerc, Arthur Zebré, Jérémy Enault, Pierre-Jean Racine, Sandra Leblanc, Nathalie Connil, Pascal Svinareff and Marc G.J. Feuilloley. 2017. Evaluation of Streptomyces Common Scab Toxins Diffusion in Potato Tubers and through the Intestinal Barrier.Int.J.Curr.Microbiol.App.Sci. 6(5): 1662-1676. doi: https://doi.org/10.20546/ijcmas.2017.605.181