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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 534-547
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.063


Biodegradation of Diazo Dye, Reactive Blue 160 by Isolate Microbacterium sp. B12 Mutant: Identification of Intermediates by LC-MS
Chetana Roat1, Avinash  Kadam2, Tallika Patel1 and Shailesh Dave1*
1Department of Microbiology & Biotechnology, University School of Sciences,Gujarat University, Ahmedabad-380 009, India
2Department of Environmental Engineering, Kyungpook National University,80 Daehak-ro, Buk-gu, Daegu 702-701, Daegu, Republic of Korea
*Corresponding author
Abstract:

A bacterial strain, B12, proficient in decolourizing commercial dye - Reactive Blue 160 (RB160) was isolated from the soil sample collected from a textile industry. Phenotypic characterization and phylogenetic analysis of the 16S rDNA sequence indicated that the bacterial strain belonged to the genus Microbacterium. Bacterial isolate B12 showed 96% decolourization of RB160 at the concentration of 250 mg/l under   microaerobic conditions with temperature 35 ± 2°C and pH 5.0 in 24 h.  The decolourization efficiency of isolate B12 was improved by using physical and chemical mutagens, the mutant strain offered 12 h reduction in time required for the complete decolourization by wild strain when exposed to 30 sec of UV radiation and 0.5µg/ml of acridine orange. The laccase and reductase enzymes cleaved RB160 and metabolised the degraded product, the different intermediates were identified using HPLC and LC-MS.  The phytotoxicity studies confirmed that intact RB160 was more toxic than dye degraded intermediates. Microbacterium sp. B12 was found competent to degrade RB160 containing textile industry effluents showing the usefulness of the organism for possible commercial application.


Keywords: Microbacterium sp., Azo dye, Reactive Blue 160, LC-MS, Random mutagenesis.
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How to cite this article:

Chetana Roat, Avinash  Kadam, Tallika Patel and Shailesh Dave. 2016. Biodegradation of Diazo Dye, Reactive Blue 160 by Isolate Microbacterium sp. B12 Mutant: Identification of Intermediates by LC-MS.Int.J.Curr.Microbiol.App.Sci. 5(3): 534-547. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.063