National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Traditional medicinal plants are widely used for their therapeutic properties, yet scientific validation of their bioactive compounds remains limited. Solanum nigrum (black nightshade) is known for its nutritional and medicinal properties, including antagonistic potential. This study investigates the antagonistic effects of Solanum nigrum leaf extracts against Bacillus sp. and Staphylococcus sp. using different solvents. Bacterial antagonism was evaluated by inoculating the extracts into sterile nutrient broth containing bacterial cultures. Growth inhibition was assessed using a colorimeter at 600 nm, with absorbance values indicating bacterial activity. Growth promoting assay was performed to understand the effect of extract on beneficial microbial growth. A positive control (bacterial growth without extract) and a negative control (no bacterial growth) were maintained for comparison. Cold water extracts exhibited minimal inhibitory effects on bacterial growth. Methanol extracts demonstrated significant antagonistic activity, with reduced absorbance values for both bacterial strains. Dichloromethane extracts exhibited the highest antagonistic potency, with complete inhibition observed at higher concentrations. The results suggest that non-polar compounds in Solanum nigrum are more effective in bacterial inhibition. Similarly, the plant extract showed growth promoting activity for Actinomycetes an Bacillus subtilis. Solanum nigrum exhibits strong antagonistic activity, particularly in methanol and dichloromethane extracts, indicating the presence of bioactive compounds with antimicrobial properties. These findings support the plant’s potential as a natural antagonistic agent. Further studies should focus on isolating potent bioactive compounds using chromatography and exploring their applications in pharmaceutical and therapeutic fields.
Akubugwo, I. E., Obasi, A. N., & Ginika, S. C. (2007). Nutritional potential of the leaves and seeds of black nightshade-Solanum nigrum L. Var virginicum from Africa-Nigeria. Pakistan journal of Nutrition, 6(4), 323-326. http://dx.doi.org/10.3923/pjn.2007.323.326
Aouadi, A., Saoud, D. H., Rebiai, A., Ibrahim, M. H., Messaoudi, M., Alia, K.,... & Abd El-Mordy, F. M. (2024). Chemical composition’s effect on Solanum nigrum Linn.’s antioxidant capacity and erythrocyte protection: Bioactive components and molecular docking analysis. Open Life Sciences, 19(1), 20220944. https://doi.org/10.1515/biol-2022-0944
Asante, J. O., Oduro, I., Wireko-Manu, F., & Larbie, C. (2024). Assessment of the antioxidant and nutritive profile of the leaves and berries of Solanum nigrum and Solanum torvum Swart. Applied Food Research, 4(2), 100438. https://doi.org/10.1016/j.afres.2024.100438
Campisi, A., Acquaviva, R., Raciti, G., Duro, A., Rizzo, M., & Santagati, N. A. (2019). Antioxidant activities of Solanum nigrum L. leaf extracts determined in in vitro cellular models. Foods, 8(2), 63. https://doi.org/10.3390/foods8020063
Compant, S., Duffy, B., Nowak, J., Clément, C., & Barka, E. A. (2005). Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Applied and environmental microbiology, 71(9), 4951-4959. https://doi.org/10.1128/aem.71.9.4951-4959.2005
Djaafar, Z., & Ridha, O. M. (2014). Phytochemical Study of Selected Medicinal plant, Solanum nigrum, the Algerian Desert. International Letters of Chemistry, Physics and Astronomy, Vol. 1, 25–30. http://dx.doi.org/10.18052/www.scipress.com/ILCPA.20.25
Gafforov, Y., Rašeta, M., Zafar, M., Makhkamov, T., Yarasheva, M., Chen, J.-J., Zhumagul, M., Wang, M., Ghosh, S., Abbasi, A. M., Yuldashev, A., Mamarakhimov, O., Alosaimi, A. A., Berdieva, D., & Rapior, S. (2024). Exploring biodiversity and ethnobotanical significance of Solanum species in Uzbekistan: Unveiling the cultural wealth and ethnopharmacological uses. Frontiers in Pharmacology, 14. https://doi.org/10.3389/fphar.2023.1287793
Hameed, I. H., Cotos, M. R. C., & Hadi, M. Y. (2017). A Review: Solanum nigrum L. Antimicrobial, Antioxidant properties, Hepatoprotective effects and Analysis of Bioactive Natural Compounds. Research Journal of Pharmacy and Technology, 10(11), 4063. https://doi.org/10.5958/0974-360X.2017.00737.5
Jahnen-Dechent, W., & Ketteler, M. (2012). Magnesium basics. Clinical kidney journal, 5(Suppl_1), i3-i14. https://doi.org/10.1093/ndtplus/sfr163
Kolar, K. (1992). Gravimetric determination of moisture and ash in meat and meat products: NMKL interlaboratory study. Journal of AOAC International, 75(6), 1016-1022. https://doi.org/10.1093/jaoac/75.6.1016
Kumar, P., Kumar, J., Kumar, R., & Dubey, R. C. (2016). Studies on phytochemical constituents and antimicrobial activities of leaves, fruits and stems of Solanum nigrum L.
Mukherjee, G., Saha, C., Naskar, N., Mukherjee, A., Mukherjee, A., Lahiri, S., Majumder, A. L., & Seal, A. (2018). An Endophytic Bacterial Consortium modulates multiple strategies to improve Arsenic Phytoremediation Efficacy in Solanum nigrum. Scientific Reports, 8(1), 6979. https://doi.org/10.1038/s41598-018-25306-x
Nielsen, S. S. (2009). Phenol-sulfuric acid method for total carbohydrates. In Food analysis laboratory manual (pp. 47-53). Boston, MA: Springer Us. https://doi.org/10.1007/978-1-4419-1463-7_6
Padmashree, A., Sharma, G. K., Semwal, A. D., & Mahesh, C. (2014). Antioxygenic activity of Solanum nigrum L. leaves in sunflower oil model system and its thermal stability. Food and Nutrition Sciences, 5(11), 1022-1029. http://dx.doi.org/10.4236/fns.2014.511113
Pallab, K., Tapan, B., Tapas, P., & Ramen, K. (2013). Estimation of total flavonoids content (TPC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug delivery and Therapeutics, 3(4), 33-37. https://jddtonline.info/index.php/jddt/article/view/546
Pathakakula, S., Lakshmanan, N., Thiruvenkatasamy, S., Subramaniam, G., Boopathi Narayanan, M., & Kalichamy, C. (2024). Mutation Breeding on Medicinal Solanum Species: Advances, Applicability and Challenges. Plant Breeding. https://doi.org/10.1111/pbr.13253
Rono, R. C. (2018). Genetic diversity and morphological characterization of African nightshade entries (section Solanum L.). https://repo.uni-hannover.de/handle/123456789/4241
Saibu, G., Adeyemo, G., Adu, O., Oguntibeju, O., Oyedeji, O., Oyedeji, A. O., & Nakin, V. (2021). Proximate and Phytochemical analysis of Aqueous Leaf Extract of Solanum nigrum from Lagos, South-West Nigeria. African Journal of Biomedical Research, 24(3), 397-402.
Sangija, F., Martin, H., & Matemu, A. (2021). African nightshades (Solanum nigrum complex): The potential contribution to human nutrition and livelihoods in sub-Saharan Africa. Comprehensive Reviews in Food Science and Food Safety, 20(4), 3284–3318. https://doi.org/10.1111/1541-4337.12756
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., & Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiological research, 183, 92-99. https://doi.org/10.1016/j.micres.2015.11.008
Schillinger, U., & Lücke, F. K. (1989). Inhibiting salmonellae growth in fresh, spreadable mettwurst (dry sausage eaten relatively fresh) made without sugar. 879-882. cabidigitallibrary.org
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in enzymology (Vol. 299, pp. 152-178). Academic press. http://dx.doi.org/10.1016/S0076-6879(99)99017-1
Sunitha, J., Krishna, S., Ananthalakshmi, R., Jeeva, J. S., Girija, A. S., & Jeddy, N. (2017). Antimicrobial Effect of Leaves of Phyllanthus niruri and Solanum nigrum on Caries Causing Bacteria: An In vitro Study. Journal of Clinical and Diagnostic Research?: JCDR, 11(6), KC01–KC04. https://doi.org/10.7860/JCDR/2017/23602.10066
Taghavi, S., van der Lelie, D., Hoffman, A., Zhang, Y. B., Walla, M. D., Vangronsveld, J.,... & Monchy, S. (2010). Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638. PLoS genetics, 6(5), e1000943. https://doi.org/10.1371/journal.pgen.1000943
Waterborg, J. H., & Matthews, H. R. (1994). The Lowry method for protein quantitation. Basic protein and peptide protocols, 1-4.
Wolters, M. G., Verbeek, C., Van Westerop, J. J., Hermus, R. J., & Voragen, A. G. (1992). Comparison of different methods for determination of dietary fiber. Journal of AOAC International, 75(4), 626-634.
Citations |
 |
 |
 |
 |
