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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1538-1552
DOI: https://doi.org/10.20546/ijcmas.2017.605.168


Production of Alkaline Keratinolytic Protease by Bacillus sp. B13 from Feather Waste
Shilpa Ashok Jani1*, Sumaiya Malek2, Anvi Patel3, Krupa Pathak2 and Kinjal Baria2
1Department of Microbiology, J and J College of Science, Nadiad,Gujarat University, Gujarat, India
2Department of Microbiology, Gujarat Science College, Ellisbridge,
Ahmedabad, Gujarat, India
3Shri P.M. Patel Institute of P.G. studies and research in science, Anand, Gujarat, India
*Corresponding author
Abstract:

Alkaline keratinolytic protease producing Bacillus species was screened out from soil sample and characterized by studying morphological, cultural and biochemical properties. It was identified by using Burgey’s manual of determinative bacteriology, identification flow charts as Bacillus alcalophilus. The isolate was employed for the production of alkaline protease by using production medium consisting of glucose and casein as carbon and nitrogen sources at pH 8.5 (Rao and Narasu, 2007). The isolate was also checked for its protease production by using feathers as sole source of carbon and nitrogen and produced significant amount of alkaline keratinolytic protease from feathers. The production was optimized for different criteria like, temperature, pH of production medium, substrate concentration (feather), incubation period of production, inoculums size. The alkaline protease produced from feather waste was also checked for it’s detergent compatibility and washing performance. It was found most compatible with detergent Wheel retaining 99.46% activity and with remarkable washing performance hence the enzyme can be used as detergent additive.


Keywords: Keratinolytic, Bacillus spp, Keratinolytic alkaline protease, Feather degradation
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How to cite this article:

Shilpa Ashok Jani, Sumaiya Malek, Anvi Patel, Krupa Pathak and Kinjal Baria. 2017. Production of Alkaline Keratinolytic Protease by Bacillus sp. B13 from Feather Waste.Int.J.Curr.Microbiol.App.Sci. 6(5): 1538-1552. doi: https://doi.org/10.20546/ijcmas.2017.605.168