National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Alkaline keratinolytic protease producing Bacillus species was screened out from soil sample and characterized by studying morphological, cultural and biochemical properties. It was identified by using Burgey’s manual of determinative bacteriology, identification flow charts as Bacillus alcalophilus. The isolate was employed for the production of alkaline protease by using production medium consisting of glucose and casein as carbon and nitrogen sources at pH 8.5 (Rao and Narasu, 2007). The isolate was also checked for its protease production by using feathers as sole source of carbon and nitrogen and produced significant amount of alkaline keratinolytic protease from feathers. The production was optimized for different criteria like, temperature, pH of production medium, substrate concentration (feather), incubation period of production, inoculums size. The alkaline protease produced from feather waste was also checked for it’s detergent compatibility and washing performance. It was found most compatible with detergent Wheel retaining 99.46% activity and with remarkable washing performance hence the enzyme can be used as detergent additive.
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