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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:4, April, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(4): 115-127
DOI: https://doi.org/10.20546/ijcmas.2025.1404.015


Molecular Identification of Bacteria Isolated from Fermented Chillies (Republic of Congo)
Bikouma Charles1,3, Didine, Dianzitoukoulou Matsima Louis Donald1, Soloka Mabika Faly Armel1,3*, Nzaou Stech Anomène Eckzechel1,2,3, Mfoutou Mampanguy Claujeans Chastel1,3 and Nguimbi Etienne1,2,3
1Laboratory of Cellular and Molecular Biology, Faculty of Science and Technology,
Brazzaville, Republic of Congo
2Institut National de Recherche en Sciences Extactes Naturelles (IRSEN).
Brazzaville Republique du Congo
3Molecular Biology and Bioinformatics Unit, Faculty of Science and Technology,
Marien Ngouabi University, Brazzaville, Republic of Congo
*Corresponding author
Abstract:

The aim of this study was the molecular identification of bacteria isolated from fermented chillies in markets in Congo (Republic of Congo). Enumeration and isolation were carried out on Mossel and PCA media; amplification and sequencing of 16S RNA genes were performed by PCR and Artic Oxford Nanopore Technology. Results showed bacterial loads ranging from 3.01.104 CFU/g to 3.5.104 CFU/g on PCA and 4,7.104 CFU/g to 5.7.104 UFC/g on mossel respectively for samples 1, 2 from Brazzaville, compared with 3.3.104 UFC/g to 7.3.104 UFC/g on PCA and 7.1.104 UFC/g to 8.2.104 UFC/g for samples from nkayi. After PCR, agarose gel electrophoresis, sequencing and bioinformatics analysis, the 16S rRNA of these isolates was analyzed to identify eight (05) strains with 100% similarities, whose gene sequences were placed in GenBank to obtain corresponding accession numbers. Bacillus anthracis (ON303634), Bacillus subtilis (ON319063), Bacillus tropicus (ON319064), Bacillus cereus (ON319065), Bacillus cereus (ON319066). Phylogenetic classification was performed using Bacillus strains.


Keywords: Bacteria, PCR, sequencing, 16S rRNA


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How to cite this article:

Bikouma Charles, Didine, Dianzitoukoulou Matsima Louis Donald, Soloka Mabika Faly Armel, Nzaou Stech Anomène Eckzechel, Mfoutou Mampanguy Claujeans Chastel and Nguimbi Etienne. 2025. Molecular Identification of Bacteria Isolated from Fermented Chillies (Republic of Congo).Int.J.Curr.Microbiol.App.Sci. 14(4): 115-127. doi: https://doi.org/10.20546/ijcmas.2025.1404.015
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