![]() |
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Research was carried out on Isolation and Study of Biodegradation Capability of Hydrocarbanoclastic Bacteria from Waste Engine Oil Impacted Soils of Auto Nagar, Guntur, Andhra Pradesh. Soil samples are collected from the waste engine oil contaminated sites of Auto Nagar, Guntur, Andhra Pradesh. Soil samples was enriched with BH medium and bacterial cultures were isolated (GAS1, GAS2, GAS3, GAS4, GAS5) and purified using MS medium and tested their ability to degrade various hydrocarbons (Petrol, Diesel, Paraffin oil, Benzene, Toluene, Turbine oil, Heavy machinery oil) using DCPIP method. The isolates were found satisfactory in degrading hydrocarbons among the all isolate GAS3 was more potential in degrading provided all hydrocarbons. GAS3 was selected to study the degradation of various hydrocarbons using Gravimetric analysis results showed that high potential in degradation of hydrocarbons with 90% of Turbine oil, 88% of Paraffin oil, 85% heavy machinery oil, 70% of diesel, 59% of petrol, 38% of benzene, 30% of toluene respectively. Based on the morphology potential isolate was identified as gram-negative bacterium.
Adebusoye SA, Ilori MO, Amund OO, Teniola OD, Olatope SO. Microbial degradation of petroleum hydrocarbons in a polluted tropical stream. World journal of Microbiology and Biotechnology. 2007; 23(8):1149- 1159
Bento FM, de Oliveira Camargo FA, Okeke BC, Frankenberger Jr WT. Diversity of bio-surfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiological research. 2005; 160(3):249-255.
Bharti P, Irafan M. Pseudomonas aeruginosa is present in crude oil contaminated sites of Barmer Region (India). Journal of bioremediation and biodegradation. 2011; 2(5):1-2.
Bundy JG, Paton GI, Campbell CD. Combined microbial community level and single species biosensor responses to monitor recovery of oil polluted soil. Soil Biol Biochem 2004;36:1149-59.
Das, N. and Chandran, P., 2011. Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnology Research International, 941810, 13 pgs. doi: 10.4061/2011/941810
Kumar PSV, Manjunatha BK. Isolation of Diesel, Engine Oil and Crude Oil Degrading Fungi from Oil Contaminated Soil for their Bioremediation Potential. J Agric. Food Nat. Resource. 2017; 1(1):65-70.
Lyle G,. Whyte L, Charles WG. Biodegradation of Petroleum Hydrocarbons by Psychrotrophic Pseudomonas Strains Possessing Both Alkane (alk) and Naphthalene (nah) Catabolic Pathways, Appl Environ Microbiol 1997;63: 3719 23.
Kanwal, H. Ullah, A. Gulzar, T. Sadiq, Z. Gul, M. Ullah, M. Sarfraz, M.W. Aslam, N.N. Khan, T. Batool, S. Maqsood, A. NawazBiodegradation of petroleum hydrocarbons and the factors effecting rate of biodegradationAm. J. Biom. Sci., 16 (1) (2022).
Riser-Roberts E. Bioremediation of Petroleum Contaminated Sites. Boca Raton (FL): CRC Press Inc; 1992.
Saadoun I. Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. J Basic Microbiol 2002; 42:420 8.
Vinothini C, Sudhakar S, Ravikumar. Biodegradation of petroleum and crude oil by Pseudomonas putida and Bacillus cereus. Int J Curr Microbiol App Sci. 2015; 4(1):318-329.
Gao, Z. Cui, Q. Li, G. Xu, X. Jia, L. Zheng Marinobacter nanhaiticus Sp. Nov., polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South ChinaAntonie Van Leeuwenhoek, 103 (3) (2012), pp. 485-491.![]() |
![]() |
![]() |
![]() |
![]() |