National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Endophytes are microorganisms that inhabit asymptomatically in distinct parts of the plant. Fungal endophytes are treasury of therapeutically significant bioactive compounds. Nyctanthes arbor-tristis L. is a divine tree, and important indigenous medicinal plant. It has been utilized in medicinal systems of Ayurveda, Siddha and Unani for treating several diseases. It is a store house of endophytes, that produces magnificent therapeutic compounds. The aim focuses on isolation of fungal endophyte Fusarium oxysporum from twigs of N. arbor-tristis, qualitative analysis of methanolic extracts of endophytic fungus extract, antimicrobial activity of Gram positive and Gram negative bacteria and characterization of its bioactive components through Gas Chromatography-Mass Spectrometry (GC-MS), UV-Visible spectrophotometry (UV-Vis), and Fourier Transform Infrared Spectroscopy (FTIR). Qualitative analysis of methanolic extracts of endophytic fungus confirms the presence of alkaloids, flavonoids, tannins, terpenoids, steroids, carbohydrates and amino acids. Antimicrobial activity performed against both Gram positive and Gram negative bacteria by disc diffusion method inhibited the growth of bacteria. Peaks between 190 to 1100 nm were manifested through UV visible spectrophotometry which reveals the presence of secondary metabolites in F. oxysporum. The results of FTIR-analysis validate the existence of alkenes, alkynes, amines, aromatics, nitro compounds, carboxylic acids, esters, ethers, alcohols and phenols. GC-MS analysis of methanolic extract of endophytic fungus disclosed many important bioactive compounds crucial in medical field.
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