The current study was conducted to examine the role of Jasmonic acid and Glutamine in callus growth and multiplication. Seeds were germinated after disinfect by NaOCl at 4.5% for 20 min and cultured on full strength Murashige and Skoog (1962)(MS) medium. callus was initiated from Cotyledons explants taken from seeding were cultured growth on MS medium supplemented with different concentration of growth hormone include the Cytokinins Benzyl Amino Purine (BAP) at concentration (0.0,2.0) mg/l. and Auxin Naphthalene Acetic Acid (NAA) at concentration (0.0,1.0,2.0,4.0) mg/l for both growth regulators. Depending upon the result of chemical analysis, the combination of BAP at 2.0 Mg/L with NAA at1.0 Mg/L choised and used Jasmonic acid and Glutamine with different concentration of Jasmonic acid at concentration (0.0, 4.0) mg/l and Glutamine at concentration (250,300,350,400) Mg/L for 8 week culture period in callus induction. The results showed the presence of significant differences between the treatments in the fresh and dry weight of callus after five weeks from culture. 2.0 mg/l BAP treatment was significantly superior on control treatment in fresh and dry weight of callus, which reached 0.335 and 0.160 mg, respectively. Also, the two concentrations of NAA (1.0 mg/l) were significantly superior on control treatment in the same of two characteristics (0.332 and 0.150 mg fresh weight, and dry weight, respectively). The treatment of interaction between BAP and NAA (0.2+1.0 mg.L-1) has given the highest significant difference in fresh and dry weight reached 0.483 and 0.215 mg, respectively. While less fresh and dry weight when treatment was control treatment, which reached 0.0 mg. The 4.0 mg/l Glutamine treatment was significantly superior on control treatment in fresh and dry weight of callus, which reached 290.42 and 40.855 mg, respectively. Also, the 350 mg/l concentration of Glutamine was significantly superior on control treatment in the same of two characteristics (286.38 and 54.664 mg fresh and dry weight). The treatment of interaction between Jasmonic acid and Glutamine (4.0 mg/l + 300 mg/l) has given the highest significant difference in fresh and dry weight reached 345.35 and 54.664 mg, respectively. While less fresh and dry weight when treatment 0 Jasmonic acid + 250 Glutamine mg/l concentrations, which reached 184.47 and 10.18 mg, respectively.

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