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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1406-1414
DOI: https://doi.org/10.20546/ijcmas.2017.605.153


Proximate Constitution and Antiproliferative Activity of Pleurotus opuntiae (Durieu and Lev.) Sacc. against Colon Cancer
P.J. Krishnapriya* and D. Geetha
Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Trissur, India
*Corresponding author
Abstract:

Pleurotus opuntiae owing to its short duration, pure white nature and ability to utilise the locally available substrates make it a promising oyster mushroom species, which can revolutionize the mushroom farming. The particular variety, isolated from arecanut tree (Areca catechu L.) logs was confirmed by ITS sequencing and registered at Genbank database (accession number: KY214255). Awareness of the nutritional and medicinal importance of P. opuntiae is not extensive. Proximate constituents of the harvested sporocarps, viz., moisture, carbohydrates, protein, fat, fibre, ash, lipids and polysaccharides viz., cellulose, starch, reducing sugars were analysed. The results indicated the presence of significant nutritional quantities viz., high amount of moisture (90.70%), carbohydrates (46.13%), protein (19.96%), fat (2.2%), fibre (9.68%), ash (6.09%), lipids (3.88%), cellulose (11.26), starch (7.5) and reducing sugars (6.31%). Anti-cancer activity of the mushroom, was analysed using MTT (Microculture Tetrazolium Assay), against HT-29 (human colon adenocarcinoma) cell line at five different concentrations of dried mushroom extract (6.25, 12.5, 25, 50, 100µg/ml). Dried extract of mushroom at 100µg/ml, recorded the highest anti-proliferative effect, with least absorbance (0.641) at 540 nm and minimum cell viability (29.27%). Rounding or shrinking of cells, granulation and vacuolization in the cytoplasm of the cancer cell lines were observed as indicators of cytotoxicity.


Keywords: Pleurotus opuntiae, proximate constituents, anticancer, MTT assay.
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How to cite this article:

Krishnapriya, P.J. and Geetha, D. 2017. Proximate Constitution and Antiproliferative Activity of Pleurotus opuntiae (Durieu and Lev.) Sacc. against Colon Cancer.Int.J.Curr.Microbiol.App.Sci. 6(5): 1406-1414. doi: https://doi.org/10.20546/ijcmas.2017.605.153