National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pleurotus opuntiae owing to its short duration, pure white nature and ability to utilise the locally available substrates make it a promising oyster mushroom species, which can revolutionize the mushroom farming. The particular variety, isolated from arecanut tree (Areca catechu L.) logs was confirmed by ITS sequencing and registered at Genbank database (accession number: KY214255). Awareness of the nutritional and medicinal importance of P. opuntiae is not extensive. Proximate constituents of the harvested sporocarps, viz., moisture, carbohydrates, protein, fat, fibre, ash, lipids and polysaccharides viz., cellulose, starch, reducing sugars were analysed. The results indicated the presence of significant nutritional quantities viz., high amount of moisture (90.70%), carbohydrates (46.13%), protein (19.96%), fat (2.2%), fibre (9.68%), ash (6.09%), lipids (3.88%), cellulose (11.26), starch (7.5) and reducing sugars (6.31%). Anti-cancer activity of the mushroom, was analysed using MTT (Microculture Tetrazolium Assay), against HT-29 (human colon adenocarcinoma) cell line at five different concentrations of dried mushroom extract (6.25, 12.5, 25, 50, 100µg/ml). Dried extract of mushroom at 100µg/ml, recorded the highest anti-proliferative effect, with least absorbance (0.641) at 540 nm and minimum cell viability (29.27%). Rounding or shrinking of cells, granulation and vacuolization in the cytoplasm of the cancer cell lines were observed as indicators of cytotoxicity.
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