Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1322-1330
DOI: https://doi.org/10.20546/ijcmas.2017.605.143


Prevalence of Human Immunodeficiency Virus Type 1 Drug Resistance Associated Mutations in Protease Cleavage and Non-Cleavage Sites and PTAP Motif Duplications in Indian HIV-1 Subtype C gag Regions
Sourav Sen1*, Srikanth P. Tripathy2 and Ramesh S. Paranjape3
1Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India
2National Institute for Research in Tuberculosis (Indian Council of Medical Research),Chennai, Tamil Nadu, India
3National AIDS Research Institute (Indian Council of Medical Research),Pune, Maharashtra, India
*Corresponding author
Abstract:

HIV-1 protease is responsible for total twelve proteolytic reactions that are required to generate a viable virus. Mutations in gag cleavage and non-cleavage sites have been reported to aid in recovery of diminished replication capacity due to HIV resistance mutations in protease. Duplication of HIV-1 p6 gag proline-rich (PTAP) region, though not exclusively related to drug resistance, may improve the replication of HIV-1. In subtype C, PTAP/PSAP duplications have been reported to be significantly higher than that seen in non-C subtype sequences. We analyzed Indian HIV-1 gag gene amino acid sequences from antiretroviral drug treatment-naïve and treatment-experienced study participant specimens, which included 72 proviral (subtype C = 70; subtype A1= 2) and 56 plasma (subtype C = 55; subtype A1= 1) sequences. Sequences from one treatment-naïve and two PI-exposed study participants exhibited A431V mutation in gag, a mutation associated with PI resistance. The remaining sequences harboring such PI resistance associated mutations in gag were in those who were either treatment-naïve, or had been exposed to NRTIs and NNRTIs only. In the study sequences, the frequency of PTAP/PSAP duplications ranged from 16% in plasma to 20% in proviral DNA, with no statistically significant differences in the prevalence of these duplications between sequences from treatment-naïve and treatment-experienced individuals. There is a need to explore the phenotypic behavior and clinical correlates of mutations and duplications in non-B subtypes in protease cleavage and non-cleavage sites in gag, with relevance to HIV drug resistance.


Keywords: HIV-1, Protease cleavage site mutations, p6gag motifs.
Download this article as Download

How to cite this article:

Sourav Sen, Srikanth P. Tripathy and Ramesh S. Paranjape. 2017. Prevalence of Human Immunodeficiency Virus Type 1 Drug Resistance Associated Mutations in Protease Cleavage and Non-Cleavage Sites and PTAP Motif Duplications in Indian HIV-1 Subtype C gag Regions.Int.J.Curr.Microbiol.App.Sci. 6(5): 1322-1330. doi: https://doi.org/10.20546/ijcmas.2017.605.143