Genetic variation among 21 Tuberose genotypes were evaluated using six inter simple sequence repeat (ISSR) markers. The results suggested that the ISSR markers produced much better reproducible bands and were more efficient in grouping germplasm. Polymorphic Information Content (PIC), Resolving Power (RP) and Marker Index (MI) for ISSR varied from 0.50-0.99, 1.61 - 3.80 and 0.99-4.57 respectively. The number of ISSR fragments generated per primer set ranged from 1 to 5 with fragment sizes varying from 180-1400 bp. A total of 19 polymorphic bands generated 100% polymorphism. All germplasm were clearly differentiated by their ISSR fingerprints. The Jaccard similarity indices (J) based on ISSR profiles were subjected to UPGMA cluster analysis. The dendogram generated by ISSR markers revealed three major groups and noted considerable amount of variation. Genotypes namely Hyderabad Single, Kalyani Single and Pearl double petaled cultivar were found to be more diverse in molecular analysis.

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