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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1313-1321
DOI: https://doi.org/10.20546/ijcmas.2017.605.142


Genetic Diversity in Tuberose (Polianthes tuberosa L.) Germplasm using Inter Simple Sequence Repeat (ISSR) Markers
Ujjwal Sirohi, Mukesh Kumar1*, Pankaj Chauhan, Navneet Kumar2, S. Prakash1,
Pooran Chand2, R.K. Naresh3, V. Rakesh Sharma1 and Veena Chaudhary4
1Department of Horticulture
2Department of Genetics and Plant Breeding
3Department of Agronomy
4Department of Chemistry, CSSS (PG) College,Machhra, Meerut, UP, India
*Corresponding author
Abstract:

Genetic variation among 21 Tuberose genotypes were evaluated using six inter simple sequence repeat (ISSR) markers. The results suggested that the ISSR markers produced much better reproducible bands and were more efficient in grouping germplasm. Polymorphic Information Content (PIC), Resolving Power (RP) and Marker Index (MI) for ISSR varied from 0.50-0.99, 1.61 - 3.80 and 0.99-4.57 respectively. The number of ISSR fragments generated per primer set ranged from 1 to 5 with fragment sizes varying from 180-1400 bp. A total of 19 polymorphic bands generated 100% polymorphism. All germplasm were clearly differentiated by their ISSR fingerprints. The Jaccard similarity indices (J) based on ISSR profiles were subjected to UPGMA cluster analysis. The dendogram generated by ISSR markers revealed three major groups and noted considerable amount of variation. Genotypes namely Hyderabad Single, Kalyani Single and Pearl double petaled cultivar were found to be more diverse in molecular analysis.


Keywords: Genetic diversity, Polianthes tuberosa, ISSR, Tuberose germplasm.
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How to cite this article:

Ujjwal Sirohi, Mukesh Kumar, Pankaj Chauhan, Navneet Kumar, S. Prakash, Pooran Chand, R.K. Naresh, V. Rakesh Sharma and Veena Chaudhary. 2017. Genetic diversity in tuberose (Polianthes tuberosa L.) germplasm using Inter Simple Sequence Repeat (ISSR) markersInt.J.Curr.Microbiol.App.Sci. 6(5): 1313-1321. doi: https://doi.org/10.20546/ijcmas.2017.605.142