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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1260-1267
DOI: https://doi.org/10.20546/ijcmas.2017.605.136


Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting
M.K. Yadav1*, Ram Chandra2, ­H.B. Singh2, S.K. Yadav4, S.K. Yadav3, Sushreeta Naik2 and P.K. Dhakad2
1Rani Lakshmi Bai Central Agricultural University, Jhansi-284003 (U.P.), India
2Department of Mycology and Plant Pathology,
3Department of Agricultural Entomology and Zoology,
4Department of Botany, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005 (U.P.), India
*Corresponding author
Abstract:

Wild edible mushroom are the special product of the forest. The diversity of mushroom and their natural beauty inhabit major threat in the biological world and India has been a frame for these fungi. The previous attempts for genetic improvement in mushrooms had little success due to low genetic diversity amongst commercially cultivated mushrooms strains and non- inclusion of wild collections in the breeding programs. This limited availability of genetic variation significantly slowed down the progress of genetic improvement in this commercially most important mushroom. The present investigation of genetic diversity of Pleurotus species were ranged from 30 to 70%. The Cluster analysis were using Unweighted Pair-Group Method with Arithmetic Mean (UPGMA), clearly separate dendrogram was obtained from the molecular marker systems for all the eight Pleurotus species. Clustering on the basis of Random Amplified Polymorphic DNA (RAPD) marker using Jaccard’s coefficient generated two (I and II) broad groups of six and two Pleurotus species. Clusters also confirmed 70% of genetic diversity among the Pleurotus species.


Keywords: Clusters, Dendrogram, Genetic diversity, Molecular marker, Pleurotus.
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How to cite this article:

Yadav, M.K., Ram Chandra, H.B. Singh, S.K. Yadav, S.K. Yadav, Sushreeta Naik and Dhakad, P.K. 2017. Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting.Int.J.Curr.Microbiol.App.Sci. 6(5): 1260-1267. doi: https://doi.org/10.20546/ijcmas.2017.605.136