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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1173-1190
DOI: https://doi.org/10.20546/ijcmas.2017.605.128


Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Solanaceae Crops
Sujeet Kumar1*, Kedarnath2, N. Hamsaveni1, P.H. Ramanjini Gowda1 I.B. Rohini1, K.T. Rangaswamy2 and Raghavendra Achari2
1Department of Plant Biotechnology and
2Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru-560065, India
*Corresponding author
Abstract:

Bacterial wilt caused by Ralstonia solanacearum is the most destructive disease of crop plants. Six isolates of R. solanacearum causing wilt disease in tomato (Solanum lycopersicum) and brinjal (S. melongena) were collected from different locations of Karnataka, India. All the six isolates were identified as R. solanacearum by colonies phenotype, simple staining, streaming and R. solanacearum species specific PCR. In this study, six isolates were differentiated into race on the basis of their ability to infect different host. The isolates-1, 2, 4 and 5 were established as race-3 and isolates-3 and 6 were established as race-1. Biovar characterization was performed by their ability to utilize disaccharides [Sucrose, lactose, and maltose (Himedia)] and sugar alcohols [mannitol, sorbitol and dulcitol (Himedia)]. According to Hayward`s classification system, the isolates-1, 2, 4 and 5 were belongs to biovars-2 and isolates-3 and 6 were belongs to biovars-3. Molecular analysis, including multiplex PCR-based phylotyping and sequence analysis of 16s rDNA were used to determine the ITS sequences of six R. solanacearum strains. The results of Pmx-PCR for all six isolates revealed that all isolates are belonged to phylotype I. Therefore, it may be confirmed that R. solanacearum causing bacterial wilt of tomato and brinjal in Karnataka belong to Biovar-2, 3 and Race 1, 3 and phylotype I.


Keywords: Ralstonia solanacearum, Bacterial wilt, Tomato, Phylotype, Biovar.
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How to cite this article:

Sujeet Kumar, Kedarnath, N. Hamsaveni, I.B. Rohini, K.T. Rangaswamy, P.H. Ramanjini Gowda and Raghavendra Achari. 2017. Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Solanaceae CropsInt.J.Curr.Microbiol.App.Sci. 6(5): 1173-1190. doi: https://doi.org/10.20546/ijcmas.2017.605.128