Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1150-1158
DOI: https://doi.org/10.20546/ijcmas.2017.605.125


Assessment of Genetic Diversity Using ISSR Markers in Green Gram [Vigna radiata (L.) Wilczek]
Anamika Nath, S.R. Maloo, B.L. Meena*, A. Gangarani Devi and Sheetal Tak
Rajasthan College of Agriculture, MPUAT, Udaipur (Rajasthan) -313001, India
*Corresponding author
Abstract:

Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied methods is ISSR marker analysis. ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes. Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement. ISSR marker analysis was performed to detect relatedness and diversity among eight parental genotypes. ISSR markers are useful in detecting polymorphism among accessions by generating a large number of markers that target multiple microsatellite loci distributed across the genome. Out of 109 scorable bands, 88 bands were polymorphic and the level of polymorphism was 81 per cent. Twenty five ISSR primers were used, out of which eighteen primers showed amplification in all genotypes. The average number of bands per primer was found to be 6.22 and average numbers of polymorphic bands per primer were 4.89. ISSR-01 proved to be best primer in the present investigation with total 29 fragments and eight highest scorable bands as well as 100 per cent polymorphism.


Keywords: Mungbean, ISSR Markers, Yield and yield components.
Download this article as Download

How to cite this article:

Anamika Nath, S.R. Maloo, B.L. Meena, A. Gangarani Devi and Sheetal Tak. 2017. Assessment of Genetic Diversity Using ISSR Markers in Green Gram [Vigna radiata (L.) Wilczek].Int.J.Curr.Microbiol.App.Sci. 6(5): 1150-1158. doi: https://doi.org/10.20546/ijcmas.2017.605.125