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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1129-1133
DOI: https://doi.org/10.20546/ijcmas.2017.605.122


Lymphocyte Activation by IL28B Protein in Mice
S. Barathiraja*, N. Vijay, Brijesh Kumar, Lijo john, V. Umapathi, M. Sedhumadhavan and G.R. Reddy
ICAR-Indian Veterinary research Institute, Hebbal, Bengaluru-560024, India
*Corresponding author
Abstract:

Interleukin 28B (IL28B) which is also called as Interferon lambda3 (IFNλ3) has been analyzed for its immunomodulatory activity in mice. Bovine IL28B protein was administered to mice intramuscularly and then the spleenocytes were harvested from mice on 7th day post injection. The collected spleenocytes were analyzed for the activation of B and T lymphocytes using Fluorescent Activated Cell Sorting (FACS) analysis. Helper T cell activation using CD3-FITC, CD4-PE/Cy7 and CD69-PE; cytotoxic T cell activation using CD3-FITC, CD8-PE/Cy7 and CD69-PE and B cell activation using CD19-PE and CD22-FITC was studied. The results show that the IL28B protein activates both B and T lymphocytes. These immunomodulatory activities will have an added value in addition to its antiviral activity to elicit better immune response.


Keywords: Interleukin 28B (IL28B), mice, Spleenocytes, Lymphocytes, Fluorescent Activated Cell Sorting (FACS).
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How to cite this article:

Barathiraja, S., N. Vijay, Brijesh Kumar, Lijo john, V. Umapthi, M. Sedhumadhavan and Reddy, G.R.. 2017. Lymphocyte Activation by IL28B Protein in Mice.Int.J.Curr.Microbiol.App.Sci. 6(5): 1129-1133. doi: https://doi.org/10.20546/ijcmas.2017.605.122