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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1057-1066
DOI: https://doi.org/10.20546/ijcmas.2017.605.115


An Evaluation of Antibiotic Profile, Molecular Characterisation and Risk Factors Associated with Carbapenem Resistant Non Fermentative Gram Negative Isolates in a Tertiary Care Centre
N. Grover1, N.K. Das2*, M. Kumar3, R. Sriram1, V.L. Dudhat4, S. Prasanna5 and P. Pandit6
1Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India
2Department of Microbiology, Dr D Y Patil Vidyapeeth Medical College,Pimpri, Pune-411018, India
3Department of Lab Sciences & Molecular Medicine, Army Hospital R & R, Delhi, India
4Department of Microbiology, Microbiology and HIC Sahyadri Speciality labs, Pune, India
5Department of Microbiology, Shri Sathya Sai Medical College and Research Institute, Ammapettai, Kancheepuram- 608103, India
6Gd Spl (Microbiology) Command Hospital, Kolkata, India
*Corresponding author
Abstract:

Non-fermentative Gram negative bacteria (NFGNB) can cause serious infections in hospitalised patients. There has been an increase in resistance to carbapenems which is worrying as they are considered as antibiotics of last resort. Carbapenemases are responsible for carbapenem resistance. Study was undertaken to evaluate antibiotic profile, to ascertain risk factors associated and to detect genes responsible for carbapenem resistance in NFGNB isolates from acute wards of a tertiary care centre. The study was carried out in an urban tertiary care centre. Samples were collected from patients of acute wards and relevant clinical history was collected. Imipenem resistance detection and antibiotic susceptibility was done. A multiplex PCR was done on imipenem resistant isolates for detection of resistant genes. A total of 296 isolates were collected. Acinetobacter baumannii (132) followed by Pseudomonas aeruginosa (121) were the predominant isolates. OXA-51(72) and NDM were the predominant genes detected in Imipenem resistant A. baumannii and Pseudomonas aeruginosa (39). The carbapenem resistance in NFGNB in our hospital setting is mostly because of VIM, NDM, OXA-23, OXA-51. Constant monitoring of the incidence of such organisms in critical areas of the hospital, prompt recognition and getting rid of them is the only important preventive strategy.


Keywords: Antibiotic profile, Molecular characterisation, Carbapenem resistant non fermentative Gram negative bacteria
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How to cite this article:

Grover, N., N.K. Das, M. Kumar, R. Sriram, V.L. Dudhat, S. Prasanna and Pandit, P. 2017. An Evaluation of Antibiotic Profile, Molecular Characterisation and Risk Factors Associated with Carbapenem Resistant Non Fermentative Gram Negative Isolates in a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 6(5): 1057-1066. doi: https://doi.org/10.20546/ijcmas.2017.605.115