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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1052-1056
DOI: https://doi.org/10.20546/ijcmas.2017.605.114


Multiplex PCR for Detection of Genes Encoding Antibiotic Resistance in Staphylococcus aureus
S. Wilfred Ruban1, P. Raja2, Nithya Quintiol3, Govind Vemala4 and K. Porteen5*
1Department of Livestock Products Technology, Veterinary College, Hebbal, Bangalore,
2Department of Animal Biotechnology,
3Department of Veterinary Public Health and Epidemiology, RIVER, Pondicherry and
4Department of Livestock Products Technology (Meat Science),
 5Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, India
*Corresponding author
Abstract:

A study was carried out to evaluate the prevalence of genes encoding antibiotic resistance for commonly used antibiotics in poultry production in S. aureus isolated from Chicken meat marketed in Chennai City. A multiplex PCR was used for screening of 50 S. aureus isolates for the presence of five genes viz., aacA-aphD (aminoglycoside), tetK and tetM (tetracycline resistance), erm(A) and erm(C) (macrolide-lincosamide-steptogramin B resistance). The results of the study revealed that of the 50 isolates screened all the isolates (100 per cent) carried either one of these resistance gene. Among the various genes either alone or in combination 48 isolates (96 %) carried tetK gene, 44 isolates (88 %) carried aacA-aphD gene, 43 isolates (86 %) carried erm(C) gene, three isolates (6 %) carried tetM gene and none of the isolates carried erm(A) gene. It was evident that 37 of the isolates (74 %) carried three resistant genes followed 6 isolates with two resistance genes (12 %) and one isolate with only one gene. The study clearly indicated the prevalence of multidrug resistant S. aureus in retail chicken meat and its potential in transference of antibiotic resistance to the consumers. This multiplex PCR can be used as a simple, rapid and accurate assay for identification of antibiotic resistance profile and could be used for surveillance in epidemiological studies.


Keywords: S. aureus, Chicken meat, Antibiotic resistance gene, PCR.
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How to cite this article:

Wilfred Ruban, S., P. Raja, Nithya Quintiol, Govind Vemala and Porteen, K. 2017. Multiplex PCR for Detection of Genes Encoding Antibiotic Resistance in Staphylococcus aureusInt.J.Curr.Microbiol.App.Sci. 6(5): 1052-1056. doi: https://doi.org/10.20546/ijcmas.2017.605.114