The investigations were conducted with the using of 15 F1 hybrids developed through diallel excluding reciprocals with 6 parents of pumpkin. The germplasms were evaluated for seven biochemical traits, viz., dry matter content, total soluble solids, total sugars, reducing sugars, non-reducing sugars, ascorbic acid content and β-carotene. The analysis of variance revealed wide range of variation among genotypes for all the traits. On the basis of mean performance the hybrids namely, P2× P4 and P3× P5 for dry matter content, P5× P6 and P1× P5 for total soluble solids, P2× P3 and P1× P4 for total sugars (%), P2× P5 and P3× P5 for reducing sugars (%), P1× P3 and P2× P3 for non-reducing sugars (%), P1× P2 and P4× P6 for ascorbic acid (mg/100g) and P2× P6 and P1× P2 for β-carotene were first and second ranker on the basis of merit during pooled analysis respectively. The study concluded that these hybrids can be exploited for cultivation.

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