Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 925-933
DOI: https://doi.org/10.20546/ijcmas.2017.605.102


In vitro Androgenesis in Capsicum (Capsicum annuum L.)
Vivek Hegde1*, P.S. Partap2, R.C. Yadav3 and K.S. Baswana2
1Division of Crop Improvement, ICAR- Central Tuber Crops Institute,Thiruvananthapuram, Kerala, India
2Department of Vegetable Science, 3Department of Molecular Biology and Biotechnology
CCS Haryana Agricultural University, Hisar-125 004, Haryana, India
*Corresponding author
Abstract:

Experiment on anther culture in sweet pepper (Capsicum annuum L.) hybrids Bharat and Indra was carried out to investigate the responses of three anther developmental stages on the production of androgenic/microspore derived calli, using MS medium supplemented with various growth regulators in different ratios and with or without pre-culture heat treatment of anthers at 35oC for seven days. In general, heat treated anthers resulted in increased callus induction percentage in all growth regulator combinations and flower bud developmental stages. Most promising results were obtained on MS medium supplemented with 1.0 mg/l zeatin along with 0.2 mg/l 2, 4-D and 15.0 mg/l AgNO3 with heat treatment reported maximum callus induction in both the hybrids, 54.02% and 60.92%, respectively when the anthers were at developmental stage-II (i.e. microspores at late uni-nucleate stage). Irrespective of anther developmental stage, zeatin was found superior in callus induction from cultured anthers followed by TDZ and Kinetin. Response of BAP was noted poor as compared to other cytokinins tested. The anther containing microspore at late uni-nucleate stage i.e. stage-II recorded maximum callus induction followed by anthers at stage-I having microspore at early uni-nucleate stage. The anthers at developmental stage-III, which contained mature pollen grains, were poor in callus production. Supplementation of MS medium with AgNO3 15.0 mg/l along with other growth regulators gave better results in all the stages of anther development.


Keywords: Capsicum Anther culture, Androgenesis, Doubled Haploid, Anther culture
Download this article as Download

How to cite this article:

Vivek Hegde, P.S. Partap, R.C. Yadav and Baswana, K.S. 2017. In vitro Androgenesis in Capsicum (Capsicum annuum L.).Int.J.Curr.Microbiol.App.Sci. 6(5): 925-933. doi: https://doi.org/10.20546/ijcmas.2017.605.102