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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 848-856
DOI: https://doi.org/10.20546/ijcmas.2017.605.095


Screening of Sunflower (Helianthus annuus L) Genotypes for Moisture Stress Tolerance using PEG-6000
Sheshaiah1*, I. Shankergoud2, R. Siddhesh, Nadkarni3 and Vikas V. Kulakarni4
1Department of Genetics and Plant Breeding, CoA, UAHS, Shivamogga-577225, India
2Directorate of Research, University of Agricultural Sciences, Raichur-584 104, India
3Department of Genetics and Plant Breeding, College of agriculture Vellayani, Kerala Agricultural University, Trivedrum-695522, India
4Scientist (Breeder), AICRP on Sunflower, MARS, UAS, Raichur-584 104, India
*Corresponding author
Abstract:

An in-vitro experiment was conducted using Polyethylene glycol-6000 (PEG-6000) at MARS, UAS, Raichur, to identify genotypes which overcome moisture stress condition at germination and seedling establishment stage of Sunflower based on stress tolerance index. A total of 160 sunflower genotypes in two replications were screened in petri-dishes at six water stress levels (0, 10, 15, 20, 25 and 30% of PEG-6000) induced by PEG-6000 in two factorial CRD fashion. The germination percentage, shoot length, root length and seedling length were recorded on seven days after of sowing, later these observations are converted into the germination stress tolerance index (GTI), root length stress tolerance index (RLTI), plant height stress tolerance index (PHTI) and seedling length stress tolerance index (SLTI). The results of analysis of variance showed that, all treatments, genotypes and G X T interactions were significant for all the studied traits. The treatment means indicated that GTI, RLTI, PHTI and SLTI are drastically reduced with the increased percentage of PEG. The genotype x treatment interactions at 20 and 25% PEG few genotypes exhibited significant differences for studied traits, hence, these concentrations are considered as critical doses to isolate the real moisture tolerant genotypes. Based on per se mean performance for GTI, RLTI, PHTI and SLTI, there are 34 promising genotypes were identified as a moisture stress tolerant genotypes.


Keywords: PEG-6000, GTI, RLTI, PHTI and SLTI
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How to cite this article:

Sheshaiah, I. Shankergoud, R. Siddhesh, Nadkarni and Vikas V. Kulakarni. 2017. Screening of Sunflower (Helianthus annuus L) Genotypes for Moisture Stress Tolerance using PEG-6000.Int.J.Curr.Microbiol.App.Sci. 6(5): 848-856. doi: https://doi.org/10.20546/ijcmas.2017.605.095