National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Twenty Trichoderma isolates collected from different geographical locations of Tamil Nadu were studied for their genetic relationship. The results showed that the PCR-based Random Amplified Polymorphic DNA (RAPD) marker produced DNA fragments of 0.125 kb to 10 kb size with polymorphism while employing five random primers. The dendrogram constructed using Unweighted Pair Group Method of Arithmetic means (UPGMA) produced two main clusters of Trichoderma isolates. Cluster 1 bears 5 isolates with 60 per cent similarity coefficient between them and cluster 2 bears 15 isolates with 57 per cent similarity. Overall, all the isolates have about 49 per cent similarity coefficient indicating that they were genetically varied by their unique banding patterns. Such variability has opened new possibility of using most efficient and more isolates of Trichoderma in the preparation of eco-friendly bio-pesticide.
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