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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 776-778
DOI: https://doi.org/10.20546/ijcmas.2017.605.087


Large Scales of Hydrangea macrophylla Using Tissue Culture Technique
Azza M.S. Arafa1, A.A. Nower2*, Samia S. Helme1 and H.A. Abd-Elaty1
1Department of Ornamental Horticulture, Faculty of Agriculture, Cairo University Egypt
2Department of plant biotechnology, Genetic Engineering and Biotechnology
Research Institute (GEBRI), University OF Sadat City (usc), Egypt
*Corresponding author
Abstract:

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number. Using GA3 at 1.0 mg/l was a marked increase in plant length when compared with zero level (control). During the rooting stage, the medium containing 4.0 mg/l IAA with charcoal gave the longest plantlet and the highest number of leaves, while NAA at 1.0 mg/l without charcoal gave the highest number of roots and the longest root was found at 2.0 mg/l IAA without charcoal. The longest plant (6.17 cm) was achieved when peat moss: per lite (2: 1 v/v) acclimatization mixture was used compared with other mixtures.


Keywords: Hydrangea macrophylla, in vitro, BA, GA3, micropropagation, acclimatization
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How to cite this article:

Azza M.S. Arafa, A.A. Nower, Samia S. Helme and H.A. Abd-Elaty. 2017. Large Scales of Hydrangea macrophylla Using Tissue Culture Technique.Int.J.Curr.Microbiol.App.Sci. 6(5): 776-778. doi: https://doi.org/10.20546/ijcmas.2017.605.087