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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 713-721
DOI: https://doi.org/10.20546/ijcmas.2017.605.080


Speciation and Antibiotic Susceptibility Testing of Coagulase Negative Staphylococci at a Tertiary Care Teaching Hospital
Ann Mary Alex1*, C. Mahesh2 and B.V. Navaneeth2
1Department of Microbiology ESIC Model Hospital, Asramam, Kollam, Kerala, India
2Department of Microbiology ESIC-MC PGIMSR, Bengaluru, Karnataka, India
*Corresponding author
Abstract:

The aim of the study was to speciate the CoNS isolates from various clinical samples and to study their antibiotic susceptibility pattern. Over a period of 18 months from December 2013 to May 2015, 142 Coagulase negative staphylococcal isolates grown in pure culture from various clinical samples were isolated and identified by standard procedure. Speciation of CoNS was done using novobiocin resistance test, urease activity, ornithine decarboxylase, pyrrolidonyl arylamidase and aerobic acid production from mannose. Out of 142 isolates, 56.3% were S. hemolyticus, 26.8% S. epidermidis, 5.6% S. warneri, 4.9% S. saprophyticus and 3.5% S. schleiferi sub sp schleiferi. Isolation of CoNS from urine, blood, conjunctival swab and pus were 62%, 12.7%, 7.8%% and 6.3% respectively. Antibiotic susceptibility testing showed maximum resistance to penicillin (91.6%). No resistance was noted against linezolid and vancomycin. Methicillin resistance was noted in 73.9% % of the isolates; 7% of isolates showed erythromycin induced clindamycin resistance. 45% of the total CoNS isolates were found to be multidrug resistant. The increasing recognition of pathogenic potential of CoNS and emergence of drug resistance amongst them denotes the need to adopt simple laboratory procedures to identify and understand the diversity of staphylococci isolated from clinical material.


Keywords: Coagulase negative Staphylococci; MRCoNS; AST; MDRs; Clindamycin resistance; Nosocomial infections
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How to cite this article:

Ann Mary Alex, C. Mahesh and Navaneeth, B.V. 2017. Speciation and Antibiotic Susceptibility Testing of Coagulase Negative Staphylococci at a Tertiary Care Teaching Hospital.Int.J.Curr.Microbiol.App.Sci. 6(5): 713-721. doi: https://doi.org/10.20546/ijcmas.2017.605.080