International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 11, Issue:11, November, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(11): 248-254

Evaluation of Three Laboratory Methods for Diagnosis of Giardia duodenalisin children under seven years old
Evandro Chagas Institute, Ministry of Health of Brazil. Rodovia BR-316. Km 7, s/n - Levilândia. Ananindeua City, Pará State, Brasil. CEP 67030-000
*Corresponding author

Different methods are used for the diagnosis of giardiasis and need to be evaluated for their applicability in laboratory routine. This study evaluated the effectiveness of three different methods for detecting Giardia duodenalis in fecal samples from children living in a poor area. Stool samples from 89 children aged under seven years were tested for Giardia by direct parasitological examination (DPE), immunochromatographic test (ICG), and real-time quantitative PCR (qPCR). Positive result was obtained for 22 samples using DPE, 26 using qPCR, and 38 using ICG. The tests showed conflicting results with different sensitivities and specificities, with the ICG test having the highest sensitivity but lower specificity than the qPCR. However, the qPCR detected the parasite in seven additional samples. It is necessary to use alternative methods for Giardia detection. We recommend ICG for Giardia diagnosis when the material is insufficient for microscopic analysis, particularly in cases of clinical suspicion.

Keywords: Giardia, diarrhea, diagnosis, microscopic analysis, immunochromatografic test, qPCR

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How to cite this article:

Heyde Araújo Tavares, Luana da Silva Soares, Alana Luanni Messias da Silva, Patrícia Dos Santos Lobo, Dielle Monteiro Teixeira, Joana D’Arc Pereira Mascarenhas and Mônica Cristina de Moraes Silva. 2022. Evaluation of Three Laboratory Methods for Diagnosis of Giardia duodenalis in Children Under Seven Years Old.Int.J.Curr.Microbiol.App.Sci. 11(11): 248-254. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.