National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Candida albicans a polymorphic fungus which have the ability to develop as yeast, pseudohyphae and/or true hyphae forms. Candida isolates (n=34) were collected from different clinical specimens and identification to the species level was performed. Isolates were subjected to antifungal susceptibility testing against (nystatin, amphotericinB, Itraconazole, fluconazole, terbinafine, and caspofungin) and determination of their minimum inhibitory concentrations (MICs) were carried out according to the European Committee of Antimicrobial Susceptibility Testing (EUCAST, 2015) strategies. Virulence factors including secreted hydrolases (phospholipase and aspartyl protease) and biofilm production were evaluated. The hyphal form of Candida is themain role in infection process during its invasion to mucosal membrane. A group of genes participate in monitoring of hyphae formation in C. albicans, includingsap5 and hwp1.Farnesol compound is a quorum sensing molecule which prevents converting of yeast-to-hyphae form. The aim of our study was to determine farnesol effect in yeast-to-hyphae shift, and its related gene expressions in C. albicans using qRT-PCR. Real time-PCR analysis revealed that 1/2 MIC of farnesol has significantly decreased the expressionofsap5and hwp1genes in comparison to control group (p<0.05).
Citations |
 |
 |
 |
 |
