National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Eight species were isolated from soil samples collected from different places of Kalaburagi city, Karnataka, India and identified as Aspergillus terreus, A. ochraceus, A. ornatus, A. clavatus, A. flavus, A. niger, A. flavus (I) and Aspergillus sps. for the production of α- and β- amylase at four different time intervals i.e., 96h, 120h, 144h and 168h. Highest activity for enzyme was observed in presence of A. flavus continuously after 96h, 120h and 144h of incubation. β- amylase was maximum produced by A. ornatus and A. flavus after 120h of incubation. Of eight Aspergillus sps, Aspergillus ornatus and A. flavus were selected after screening for the production of α- and β- amylase. Four different concentrations i.e. 0.5, 1.0, 1.5, 2.0 and 3.0 % of carbon sources viz., glucose, sucrose, lactose and maltose were used as supplements to check their effect on enzyme production. Considerable α-amylase was produced by A. ornatus at 2.0% after 144h of incubation in the presence of glucose and β- amylase was produced at 144h in the presence of lactose. Maximum α-amylase was produced by A. flavus (1.769 U/ml) in the presence of lactose at 2.0% concentration after 144h of incubation. Effect of various nitrogen sources were tested for production of α-amylase, of which Ammonium sulphate (1.0%) was found to be effective for α-amylase production by A. ornatus (3.559 U/ml) at 144h of incubation and Casein hydrolase (2.0%) was effective at 168h by A. flavus (3.663 U/ml). A. ornatus and A. flavus can be further investigated for bulk production of α- and β- amylase.
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